Genome analysis of Desulfotomaculum kuznetsovii strain 17T reveals a physiological similarity with Pelotomaculum thermopropionicum strain SIT
- Michael Visser1,
- Petra Worm1,
- Gerard Muyzer2,
- Inês A. C. Pereira3,
- Peter J. Schaap4,
- Caroline M. Plugge1,
- Jan Kuever5,
- Sofiya N. Parshina6,
- Tamara N. Nazina6,
- Anna E. Ivanova6,
- Rizlan Bernier-Latmani7,
- Lynne A. Goodwin8, 9,
- Nikos C. Kyrpides8,
- Tanja Woyke8,
- Patrick Chain8, 9,
- Karen W. Davenport8, 9,
- Stefan Spring10,
- Hans-Peter Klenk10 and
- Alfons J. M. Stams1, 11
© The Author(s) 2013
Published: 15 April 2013
Desulfotomaculum kuznetsovii is a moderately thermophilic member of the polyphyletic spore-forming genus Desulfotomaculum in the family Peptococcaceae. This species is of interest because it originates from deep subsurface thermal mineral water at a depth of about 3,000 m. D. kuznetsovii is a rather versatile bacterium as it can grow with a large variety of organic substrates, including short-chain and long-chain fatty acids, which are degraded completely to carbon dioxide coupled to the reduction of sulfate. It can grow methylotrophically with methanol and sulfate and autotrophically with H2 + CO2 and sulfate. For growth it does not require any vitamins. Here, we describe the features of D. kuznetsovii together with the genome sequence and annotation. The chromosome has 3,601,386 bp organized in one contig. A total of 3,567 candidate protein-encoding genes and 58 RNA genes were identified. Genes of the acetyl-CoA pathway, possibly involved in heterotrophic growth with acetate and methanol, and in CO2 fixation during autotrophic growth are present. Genomic comparison revealed that D. kuznetsovii shows a high similarity with Pelotomaculum thermopropionicum. Genes involved in propionate metabolism of these two strains show a strong similarity. However, main differences are found in genes involved in the electron acceptor metabolism.
KeywordsThermophilic spore-forming anaerobes sulfate reduction autotrophic methylotrophic Peptococcaceae Clostridiales
Desulfotomaculum kuznetsovii strain 17T (VKM B-1805; DSM 6115) is a moderately thermophilic sulfate-reducing bacterium isolated from deep subsurface thermal mineral water . It grows with a wide range of substrates, including organic acids, such as long-chain fatty acids, short-chain fatty acids (butyrate, propionate, acetate), lactate, pyruvate, fumarate and succinate as well as ethanol and methanol. These substrates are degraded to CO2 coupled to sulfate reduction. The strain is also able to grow autotrophically with H2/CO2 and sulfate and to ferment pyruvate and fumarate. For growth, D. kuznetsovii has no vitamin requirement.
Desulfotomaculum is a genus of Gram-positive, spore-forming anaerobes that is phylogenetically and physiologically very diverse. The genus is poorly studied physiologically, while its members are known to play an important role in the carbon and sulfur cycle in a variety of often adverse environments. The genus is divided phylogenetically into different sub-groups [2,3]. To get a thorough understanding of the evolutionary relationship of the different Desulfotomaculum sub-groups and the physiology of the individual species, it is important to have genome sequence information. Here, we present a summary of the features of D. kuznetsovii strain 17T, together with the description of the complete genomic sequencing and annotation. Moreover, we describe a physiological and genomic comparison of D. kuznetsovii strain 17T and Pelotomaculum thermopropionicum strain SIT, because phylogenetically P. thermopropionicum is the closest related organism with validly published name that has a completely sequenced genome. However, the two strains have different physiological traits. For example, P. thermopropionicum is not able to grow by sulfate reduction, but is able to grow in syntrophy with methanogens. D. kuznetsovii lacks this ability. By comparing the genomes of the two bacteria we were able to identify the main similarities and differences.
Classification and features
Classification and general features of D. kuznetsovii DSM 6115 according to the MIGS recommendations .
Species Desulfotomaculum kuznetsovii
Type strain 17
oval, terminal or subterminal, slightly swelling the cell.
CO2 (autotrophic) and organic substrates (heterotrophic)
Sulfate-dependent growth and fermentative growth with pyruvate and fumarate.
Sulfate, thiosulfate and sulfite.
Geothermal groundwater, sediment and hot solfataric fields.
Sample collection time
1987 or before
Genome sequencing and annotation
Genome project history
Genome sequencing project information
Four genomic libraries: one Illumina shotgun library, one 454 standard library, two paired end 454 libraries
Illumina GAii, 454 Titanium
158.2 × illumina; 30.6 × pyrosequencing
VELVET version 0.7.63; Newbler version 2.3; phrap version SPS - 4.24
Gene calling method
Prodigal 1.4, GenePRIMP
Genome Database release
July 20, 2012
Genbank Date of Release
May 24, 2011
NCBI project ID
Source material identifier
Obtain insight into the phylogenetic and physiological diversity of Desulfotomacum species, and bioremediation.
Growth conditions and DNA isolation
D. kuznetsovii was grown anaerobically at 60oC in bicarbonate buffered medium with propionate and sulfate as substrates . DNA of cell pellets was isolated using the standard DOE-JGI CTAB method recommended by the DOE Joint Genome Institute (JGI, Walnut Creek, CA, USA). In short, cells were resuspended in TE (10 mM tris; 1 mM EDTA, pH 8.0). Subsequently, cells were lysed using lysozyme and proteinase K, and DNA was extracted and purified using CTAB and phenol:chloroform:isoamylalcohol extractions. After precipitation in 2-propanol and washing in 70% ethanol, the DNA was resuspended in TE containing RNase. Following a quality and quantity check using agarose gel electrophoresis in the presence of ethidium bromide, and spectrophotometric measurement using a NanoDrop ND-1000 spectrophotometer (NanoDrop® Technologies, Wilmington, DE, USA).
Genome sequencing and assembly
The genome was sequenced using a combination of Illumina and 454 sequencing platforms. All general aspects of library construction and sequencing can be found at the JGI website . Pyrosequencing reads were assembled using the Newbler assembler (Roche).
The initial Newbler assembly consisting of 81 contigs in five scaffolds was converted into a phrap  assembly by making fake reads from the consensus, to collect the read pairs in the 454 paired end library. Illumina GAii sequencing data (570.2 Mb) was assembled with Velvet  and the consensus sequences were shredded into 1.5 kb overlapped fake reads and assembled together with the 454 data. The 454 draft assembly was based on 134.6 Mb 454 draft data and all of the 454 paired end data. Newbler parameters are -consed -a 50 -l 350 -g -m -ml 20. The Phred/Phrap/Consed software package  was used for sequence assembly and quality assessment in the subsequent finishing process. After the shotgun stage, reads were assembled with parallel phrap (High Performance Software, LLC). Possible mis-assemblies were corrected with gapResolution , Dupfinisher , or sequencing cloned bridging PCR fragments with subcloning. Gaps between contigs were closed by editing in Consed, by PCR and by Bubble PCR primer walks (J.-F. Chang, unpublished). A total of 400 additional reactions and one shatter library were necessary to close gaps and to raise the quality of the finished sequence. Illumina reads were also used to correct potential base errors and increase consensus quality using a software Polisher developed at JGI . The error rate of the completed genome sequence is less than 1 in 100,000. Together, the combination of the Illumina and 454 sequencing platforms provided 188.8 × coverage of the genome. The final assembly contained 323,815 pyrosequence and 15,594,144 Illumina reads.
Genes were identified using Prodigal  as part of the Oak Ridge National Laboratory genome annotation pipeline, followed by a round of manual curation using the JGI GenePRIMP pipeline . The predicted CDSs were translated and used to search the National Center for Biotechnology Information (NCBI) nonredundant database, UniProt, TIGR-Fam, Pfam, PRIAM, KEGG, COG, and InterPro databases. Additional gene prediction analysis and functional annotation was performed within the Integrated Microbial Genomes - Expert Review (IMG-ER) platform .
Genome properties and genome comparison with other strains
% of totala
Genome size (bp)
Genome coding region (bp)
Genome G+C content (bp)
Genes in paralog clusters
Genes assigned to COGs
Genes with signal peptides
Genes with transmembrane helices
Number of genes associated with the general COG functional categories
RNA processing and modification
Replication, recombination and repair
Chromatin structure and dynamics
Cell cycle control, mitosis and meiosis
Signal transduction mechanisms
Cell wall/membrane biogenesis
Intracellular trafficking and secretion
Posttranslational modification, protein turnover, chaperones
Energy production and conversion
Carbohydrate transport and metabolism
Amino acid transport and metabolism
Nucleotide transport and metabolism
Coenzyme transport and metabolism
Lipid transport and metabolism
Inorganic ion transport and metabolism
Secondary metabolites biosynthesis, transport and catabolism
General function prediction only
Not in COGs
The genome of D. kuznetsovii has 58 RNA genes of which, three are 16S rRNA genes. This is one more than the previously described rrnA and rrnB . These two rRNA genes contained two large inserts. One at the variable 5′terminal region and one at the variable 3′terminal region. The main differences between the two rRNA genes were found in these inserts. These inserts were hypothesized to be involved in the operation of ribosomes at high temperatures. However, more research is needed to assess the function of these inserts. All three rRNA genes of D. kuznetsovii have a size of approximately 1,700 nucleotides. This suggests that the third rRNA gene might also contain inserts. Alignment of the 16S rRNA genes confirmed the presence of inserts in all three 16S rRNA genes (data not shown).
Taxonomic distribution of the top KEGG hits of D. kuznetsovii genes based on BLAST against KEGG database.†
Candidatus Desulforudis audaxviator
Thermincola potens JR
Green nonsulfur bacteria
Green sulfur bacteria
Proteins of D. kuznetsovii conserved across three related species with fully sequenced genomes†.
Subject DB Input Query
Insights into the genome
Involvement of the acetyl-coA pathway in growth with acetate and methanol
D. acetoxidans is an acetate-oxidizing Desulfotomaculum species, positioned in sub-group 1e (Figure 1), that also uses the acetyl-CoA pathway for acetate oxidation to CO2 . The genes involved in acetate oxidation in D. acetoxidans are similar to those in D. kuznetsovii, but there are some exceptions. The genome of D. acetoxidans does not contain a gene that putatively codes for acetyl-CoA synthetase, similar to D. kuznetsovii, but contains genes that putatively code for an acetate kinase and a phosphate acetyltransferase . Additionally, putative carbon-monoxide dehydrogenase complex coding genes involved in the acetyl-CoA pathway show differences between the two Desulfotomaculum species. D. kuznetsovii lacks a ferredoxin coding gene that is located between cooC (Desku_1493) and acsE (Desku_1487), which in contrast is present in the genome of D. acetoxidans (Dtox_1273). Moreover, three genes similar to heterodisulfide reductase encoding genes (Desku_1486-1484) are located upstream of acsE in D. kuznetsovii, which is not the case in the genome of D. acetoxidans.
Growth of D. kuznetsovii with methanol and sulfate was studied . In that study the activity of methyltransferase, an enzyme that is involved in methanol metabolism in methanogens and acetogens [36,37], could not be assessed, while low activities of an alcohol dehydrogenase could be measured. An alcohol dehydrogenase with a molecular mass of 42 kDa was partially purified and showed activity with methanol . The genome of D. kuznetsovii contains several alcohol dehydrogenase genes (Desku_0165, 0619, 0624, 0628, 2955, 3082) that each code for an enzyme with a size of approximately 42 kDa. In the genome, genes with similarity to those coding for a methanol methyltransferase mtaA (Desku_0050, 0055, 0060), mtaB (Desku_0051) and mtaC (Desku_0048, 0049, 0052, 0056) were also found, suggesting a methanol metabolism as described in Moorella thermoacetica . Further studies are needed to obtain information about the diversity of the methanol-degradation pathways in D. kuznetsovii.
Comparison of D. kuznetsovii and P. thermopropionicum genomes
Genes in D. kuznetsovii that are annotated as enzymes involved in propionate metabolism†.
Homologous protein in P. thermopropionicum
Succinate dehydrogenase, FeS protein
Succinate dehydrogenase, flavoprotein
Succinate dehydrogenase, cytochrome b
Succinate dehydrogenase, flavoprotein
Succinate dehydrogenase, FeS protein
Fumarase, N-terminal domain
Fumarase, C-terminal domain
Succinyl-CoA synthetase, alpha subunit
Methylmalonyl-CoA mutase, N-terminal domain
Methylmalonyl-CoA mutase, C-terminal domain
Methylmalonyl-CoA decarboxylase, alpha subunit
Methylmalonyl-CoA decarboxylase, epsilon subunit
Methylmalonyl-CoA decarboxylase, gamma subunit
Transcarboxylase 5S subunit
Pyruvate phosphate dikinase
Hydrogenase and formate dehydrogenase genes: Schut and Adams (2009)  showed that the trimeric [FeFe]-hydrogenase from Thermotoga maritima oxidizes NADH and ferredoxin simultaneously to produce H2. Similar bifurcating / confurcating [FeFe]-hydrogenases and formate dehydrogenases are present in Syntrophobacter fumaroxidans and P. thermopropionicum . Both generate NADH and ferredoxin during propionate degradation via the methylmalonyl-CoA pathway and might use confurcating hydrogenases and formate dehydrogenases to drive the unfavorable re-oxidation of NADH (E0’=-320mV) by the exergonic re-oxidation of ferredoxin (E0’=-398mV) to produce hydrogen (E0’= -414mV) or formate (E0’= -432mV) that are subsequently transferred to hydrogen and formate scavenging methanogens. Additionally, up-regulation of genes encoding hydrogenases and formate dehydrogenases in P. thermopropionicum was shown during syntrophic growth . The P. thermopropionicum genome contains three [FeFe]-hydrogenases, one [NiFe]-hydrogenase and two formate dehydrogenases. One [FeFe]-hydrogenase (PTH_0668-0670) was shown to be down-regulated during syntrophic growth, while the other two [FeFe]-hydrogenases (PTH_1377-1379 and PTH_2010-2012) were up-regulated. The two formate dehydrogenases of P. thermopropionicum (I, PTH_1711-1714 and II, PTH_2645-2649) were both up-regulated during syntrophic growth . According to TMHMM server v. 2.0  formate dehydrogenase I of P. thermopropionicum has transmembrane helices. Therefore, it might play an essential role in the interspecies transfer of reducing equivalents in syntrophic growth.
The genome of D. kuznetsovii was screened for hydrogenase and formate dehydrogenase encoding gene clusters with BLAST analysis. Pfam search  was used to identify motifs in the amino acid sequences and the TMHMM Server v. 2.0  was used to screen for transmembrane helices. The TatP 1.0 Server was used to screen for twin-arginine translocation (Tat) motifs in the N-terminus to predict protein localization in the cell . The incorporation of selenocysteine (SeCys) was examined by RNA loop predictions with Mfold version 3.2 [46, 47]. The predicted RNA loop in the 50–100 bp region downstream of the UGA-codon was compared with the consensus loop described earlier .
Apart from a possible involvement in the acetate oxidation pathway (Figure 4), it remains unclear for which purpose D. kuznetsovii uses its confurcating formate dehydrogenase and hydrogenases because our genome analysis indicates that pyruvate oxidation during propionate degradation generates formate instead of ferredoxin (Figure 6).
D. kuznetsovii is able to grow in medium without vitamins . This indicates that D. kuznetsovii is able to synthesize all the vitamins that are required for its metabolism and that vitamin synthesis genes should be present in the genome. Vitamin B12 is essential for the methylmalonyl-CoA pathway and the acetyl-coA pathway. The biosynthesis of cobalamin (vitamin B12) is known to occur from uroporphyrinogen-III to adenosylcobalamin via two possible pathways, the aerobic and anaerobic pathway of the corrinoid ring [49,50]. The D. kuznetsovii genome contains all genes needed for the anaerobic pathway: cysG A (Desku_1520), cysG B (Desku_1460, Desku_1523), cbiA (Desku_1765, Desku_2368), cbiBCDEFGHJLPT (Desku_2369, 1459, 1468, 1467, 1464, 1463, 1462, 1461, 1465, 2370 and 1466, respectively), cobalt reductase (Desku_2757), btuR (Desku_0004, 1209), cobS (Desku_2367) and cobU (Desku_2371). Moreover, D. kuznetsovii has genes to convert glutamyl tRNA to uroporphyrinogen-III, hemABCDL (Desku_1522, 1518, 1521, 1520 and 1522, respectively). The genome also contains some unassigned cobalamin synthesis genes (P47K, Desku_0046, 0053; cbiM, Desku_2905), corrinoid transport proteins (Desku_0693, 702, 2237–2239, 2902–2904, 3025–3027) and, interestingly, two cobN genes (Desku_2189, 2227), genes involved in the aerobic pathway. It is unclear why D. kuznetsovii has these cobN genes, since all anaerobic pathway genes are present in the genome, and it is unclear if the products of these two genes are used for cobalamin synthesis by D. kuznetsovii.
Other vitamin synthesis genes present in the genome of D. kuznetsovii are genes involved in biotin synthesis (vitamin H) (Desku_1295-1297, 2246–2247, 2317), nicotinamide (vitamin B3) synthesis (Desku_0433, 0614, 0662, 0815, 1248, 1417, 1472, 1499, 1925, 1951, 3103, 3121, 3227, 3228, 3231, 3246, 3337), thiamin (vitamin B1) synthesis (0372, 0543, 0545, 2253, 2363, 2639), riboflavin (vitamin B2) synthesis (Desku_1244-1247), and pantothenate (vitamin B5) synthesis (Desku_3262). The genes involved in coenzyme A production from pantothenate are also present in the D. kuznetsovii genome (Desku_1254, 1307, 3145, 3200). Moreover, genes involved in the biosynthesis of pyridoxine (vitamin B6) via the deoxyxylulose 5-phosphate (DXP) independent route were found to be in the genome (Desku_0007, 0008). These genes code for two enzymes that facilitate the conversion of glutamine to the active form of vitamin B6, pyridoxal 5′-phosphate .
Menaquinone (vitamin K) and ubiquinone (coenzyme Q10) biosynthesis is important because of the electron transport function in the membranes. The genes that code for the biosynthesis enzymes from polyprenyldiphosphate to menaquinone and ubiquinone are present in the D. kuznetsovii genome (Desku_0124, 0126, 0629, 1551–1554, 1829, 2629 and 3525), except for the genes that code for a 2-polyprenyl-6-methoxyphenol 4-monooxygenase (UbiH) and 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone hydroxylase (UbiF). Additionally, three genes (Desku_1548-1550) could be identified as putative menaquinone biosynthesis genes and are part of a menaquinone biosynthesis gene cluster (Desku_1548-1554). The products of those three genes could be involved in the reactions of the missing UbiH and UbiF encoding genes.
Folate (vitamin B9) biosynthesis is also of great importance for D. kuznetsovii, because it is an essential part of the acetyl-CoA pathway. It is involved in the transfer of one-carbon compounds and can be biosynthesized from chorismate and guanosine triphosphate (GTP) [52–55]. Both pathways use a dihydropteroate synthase to produce dihydropteroate. The genome of D. kuznetsovii contains the genes encoding the enzymes involved in the pathway from chorismate to dihydropteroate (Desku_0219, 2268–2269) and from GTP to dihydropteroate (Desku_0210, 0219–0221 and 1419). The gene encoding a phosphatase (Desku_0210) in the D. kuznetsovii genome is probably involved in the removal of phosphate groups from dihydropterine triphosphate as a substitute for an alkaline phosphatase encoding gene, which is not present in the genome. Additionally, the genome contains a bifunctional protein encoding gene (Desku_404) that is expected to be responsible for the production of dihydrofolate (DHF) and the addition of multiple glutamate moieties to DHF or tetrahydrofolate (THF). However, the D. kuznetsovii genome lacks the DHF reductase encoding gene, which is required to reduce DHF to THF. The DHF reductase encoding gene appears to be absent in many microorganisms . Levin et al. (2004) propose that in Halobacterium salinarum a dihydrofolate synthase and a dihydropteroate synthase domain is able to replace the function of the DHF reductase. Additionally, the authors show that when using a BLAST search, homologs of polypeptides can be found in organisms that lack a DHF reductase . However, BLAST results showed no homologous protein encoding gene in the genome of D. kuznetsovii (data not shown). How in D. kuznetsovii DHF is reduced to THF can currently not be deduced from the genome sequence.
The work conducted by the U.S. Department of Energy Joint Genome Institute is supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231, and was also supported by grants CW-TOP 700.55.343 and ALW 819.02.014 of the Netherlands Science Foundation (NWO) and grant 323009 of the European Research Council.
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