Complete genome sequence of Ilumatobacter coccineum YM16-304T
© The Author(s) 2013
Published: 30 July 2013
Ilumatobacter coccineum YM16-304T (=NBRC 103263T) is a novel marine actinobacterium isolated from a sand sample collected at a beach in Shimane Prefecture, Japan. Strain YM16-304T is the type strain of the species. Phylogenetically, strain YM16-304T is close to Ilumatobacter nonamiense YM16-303T (=NBRC 109120T), Ilumatobacter fluminis YM22-133T and some uncultured bacteria including putative marine sponge symbionts. Whole genome sequence of these species has not been reported. Here we report the complete genome sequence of strain YM16-304T. The 4,830,181 bp chromosome was predicted to encode a total of 4,291 protein-coding genes.
Keywordsaerobic mesophilic marine sponge
Classification and features
Classification and general features of I. coccineum strain YM16-304T
Species Ilumatobacter coccineum
Type strain YM16-304
Peptone, yeast extract
Nonami Beach, Shimane Pref., Japan
Sample collection time
Strain YM16-304T grows poorly even in artificial seawater medium supplemented with 0.5% peptone and 0.1% yeast extract under optimum growth conditions . From the genome sequence, strain YM16-304T seems to possess either deficient or unusual pathways for the synthesis of some amino acids and other essential cellular components as outlined in the later section (Primary metabolism).
Genome sequencing information
Genome project history
two plasmid libraries with average insert sizes of 1.5 kb and 6.0 kb and a fosmid library with average insert size of 38 kb
Gene calling method
Genbank Date of Release
March 16, 2013
NCBI project ID
Gi02040 (to be updated)
Source material identifier
Growth conditions and DNA isolation
I. coccineum YM16-304T cells were grown in a 20 L volume at 27°C in DifcoTM Marine broth 2216 (Beckton Dickinson). DNA was isolated from 0.5 g of wet cells by manual extraction after lysis with lysozyme and SDS.
Genome sequencing and assembly
The genome of I. coccineum YM16-304T was sequenced using the conventional whole-genome shotgun sequencing method. Plasmid libraries with average insert sizes of 1.5 kb and 6.0 kb were generated in pTS1 (Nippon Gene) and pUC118 (TaKaRa) vectors, respectively, while a fosmid library with average insert size of 38 kb was constructed in pCC1FOS (EPICENTRE) as described previously . A total of 26,592 clones (18,432, 5,376 and 2,784 clones from libraries with 1.5 kb, 6.0 kb and 38 kb inserts, respectively) were subjected to sequencing from both ends of the inserts on a ABI 3730xl DNA Analyzer (Applied Biosystems). Sequence reads were trimmed at a threshold of 20 in Phred score and assembled by using Phrap and CONSED assembly tools [12,13]. Gaps between contigs were closed by sequencing PCR products which bridge two neighboring contigs. Finally, each base of the genome was ensured to be sequenced from multiple clones either from both directions with Phrap quality score ≥ 70 or from one direction with Phrap quality score ≥40.
The complete sequence of the chromosome was analyzed using Glimmer3  for predicting protein-coding genes, tRNAscan-SE  and ARAGORN  for tRNA genes, and RNAmmer  for rRNA genes. The functions of predicted protein-coding genes were assigned manually, using the in-house genome annotation system OCSS (unpublished), in comparison with Uniprot , Interpro , HAMAP  and KEGG  databases.
Nucleotide content and gene count levels of the genome
% of totala
Genome size (bp)
DNA Coding region (bp)
DNA G+C content (bp)
Number of replicons
Genes with function prediction
Genes in paralog clusters
Genes assigned to COGs
Genes assigned Pfam domains
Genes with signal peptides
Genes with transmembrane helices
% of totala
Number of genes associated with the 25 general COG functional categories
RNA processing and modification
Replication, recombination and repair
Chromatin structure and dynamics
Cell cycle control, mitosis and meiosis
Signal transduction mechanisms
Cell wall/membrane biogenesis
Intracellular trafficking and secretion
Posttranslational modification, protein turnover, chaperones
Energy production and conversion
Carbohydrate transport and metabolism
Amino acid transport and metabolism
Nucleotide transport and metabolism
Coenzyme transport and metabolism
Lipid transport and metabolism
Inorganic ion transport and metabolism
Secondary metabolites biosynthesis, transport and catabolism
General function prediction only
Not in COGs
Strain YM16-304T lacks the dapE gene for succinyl-diaminopimelate desuccinylase (EC:126.96.36.199) in the biosynthesis pathway of lysine and diaminopimelic acids (DAPs). Instead, two candidate genes (YM304_26990 and YM304_19190) for LL-DAP aminotransferase (EC:188.8.131.52, dapL), that constitutes an alternative DAP-lysine biosynthesis pathway (DAP aminotransferase pathway [24,25]), were identified. The dapL gene is found in discrete lineages of Bacteria and Archaea, and is known to complement Escherichia coli dapD and dapE mutants, although purified proteins favor the reverse reaction rather than the synthesis of LL-DAP .
Among the genes of serine biosynthesis pathway, the serB gene for phosphoserine phosphatase (EC:184.108.40.206) was not identified by similarity searches. On the other hand, the thrH gene for phosphoserine / homoserine phosphotransferase  (EC:220.127.116.11, 18.104.22.168) was identified (YM304_28950). The possibility of using thrH gene product for serine biosynthesis instead of serB gene product was suggested.
Strain YM16-304Tseems to possess an alternative form of histidine biosynthesis pathway in which hisB gene for the synthesis of L-histidinol was replaced with the hisN gene (YM304_12240) as typically found in Corynebacterium glutamicum ATCC13032  and other actinomycetes. However, the hisE gene for phosphoribosyl-ATP pyrophosphohydrolase (EC:22.214.171.124), which is responsible for the second step in histidine biosynthesis pathway, was not identified by similarity searches.
Metabolic reconstruction based on the annotation suggested that strain YM16-304T possesses the enzymes required for the biosynthesis of saturated fatty acids, unsaturated fatty acids, branched-chain fatty acids and carotenoids. The putative carotenoid biosynthesis pathway comprises crtE (YM304_37400), crtB (YM304_37420), crtI (YM304_37410) and crtLm (YM304_23780) gene homologs, which most probably synthesizes γ-carotene from isopentenyl pyrophosphate derived from non-mevalonate pathway [28–30]. Strain YM16-304T also possesses genes homologous to crtO (YM304_25370) and crtZ (YM304_38780), which were suggested to be involved in the synthesis of ketolated carotenoid such as canthaxanthin and astaxanthin . Actual products of this pathway need to be experimentally verified.
The annotation also suggests that strain YM16-304T possesses the enzymes required for the biosynthesis of menaquinone (vitamin K), vitamin B6, nicotinate and nicotinamide, pantothenate and CoA, lipoic acid, protoheme, mycothiol and coenzyme F420, while biosynthetic pathways for folate, thiamine, riboflavin, biotin and adenosylcobalamin (coenzyme B12) are either missing or incomplete.
The phylogenetic analysis based on 16S rRNA gene sequences showed that three species in the genus Ilumatobacter were closely related to some uncultured actinobacteria including marine sponge symbionts . Marine sponges are noted as a rich source of biologically active secondary metabolites, true producers of such compound being suspected to be symbiotic bacteria [32–34]. However, only a small percentage of these symbiotic microorganisms are culturable [35,36], and genes involved in the synthesis of bioactive compounds such as polyketide synthases have often been isolated by metagenomic approaches [37,38].
The strain YM16-304T genome seemed to encode only a limited number of secondary metabolic enzymes, i.e., two type I polyketide synthases (PKS). The genome does not contain genes for type II and type III PKS nor a gene for nonribosomal peptide synthetase.
The type I PKS genes of the strain YM16-304T (YM304_13420, YM304_13410), together with the adjacent pfaD homolog (YM304_13430), most probably encode omega-3 polyunsaturated fatty acid (PUFA) synthase gene cluster. In some Gammaproteobacteria from marine sources such as Photobacterium profundum strain SS9, omega-3 polyunsaturated fatty acids such as eicosapentaenoic acid (20:5n-3; EPA) and docosahexaenoic acid (22:6n-3; DHA) are known to be synthesized by a PKS system consisting of pfaA, pfaB, pfaC and pfaD genes [39–41]. The domain organization of YM304_13420 was identical to that of the pfaA gene of P. profundum SS9. The N-terminal ketosynthase domain and the C-terminal dehydratase domains of YM304_13410 were similar to those of the pfaC gene of P. profundum, while the internal acyltransferase domain of YM304_13410 was moderately similar to that of the pfaB gene of P. profundum, representing a presumed chimeric form of PKS. As PfaB is the key enzyme determining the final product in EPA or DHA biosynthesis , the actual product of this PKS system may need to be clarified experimentally. Some PUFA-producing bacteria such as Moritella marina MP-1 [39,43] were reported to require an additional gene, pfaE, encoding a phosphopantheteinyl transferase. However, the pfaE gene was not identified in strain YM16-304T. Other classes of phosphopantheteinyl transferase (e.g. YM304_08850) may substitute the function of PfaE, similar to the case suggested in P. profundum SS9 .
Strain YM16-304 seemed to possess 13 ORFs containing LPXTG motif (InterPro ID: IPR001899), the presumed sorting signal of cell surface proteins in Gram-positive bacteria . It was reported that several cell surface proteins containing LPXTG motif act as an adhesion factor known as microbial surface components recognizing adhesive matrix molecules (MSCRAMMs) . The genome of strain YM16-304 contained extracellular polysaccharide gene cluster (YM304_29910-YM304_30490), including gene cluster for the synthesis of sialic acids (YM304_30300-YM304_30320), which are also crucial for cell adhesion . These extracellular components might serve for the bacterium to adhere to host tissues such as marine sponges.
Many marine bacteria use the Na+ cycle and require Na+ for their growth . In these bacteria, Na+ is often used in the respiratory chain, ATP synthase, flagellar rotation and solute uptake instead of H+ . Some bacteria can use both Na+ and H+ to expand the range of environments in which the bacteria can grow . Strain YM16-304 was isolated from a sand sample collected at a beach and grows optimally in marine broth media, suggesting its marine origin. However, the gene products for the respiratory chain and ATP synthase were predicted to be of the H+-dependent type by similarity search. The Na+-dependent amino acid symporters were also not identified, nor was the H+-dependent symporters.
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