Open Access

Genome sequence of the Litoreibacter arenae type strain (DSM 19593T), a member of the Roseobacter clade isolated from sea sand

  • Thomas Riedel1, 2,
  • Anne Fiebig3,
  • Jörn Petersen3,
  • Sabine Gronow3,
  • Nikos C. Kyrpides4,
  • Markus Göker3Email author and
  • Hans-Peter Klenk3
Standards in Genomic Sciences20139:9010117

DOI: 10.4056/sigs.4258318

Published: 16 October 2013

Abstract

Litoreibacter arenae Kim et al. 2012 is a member of the genomically well-characterized Rhodobacteraceae clade within the Roseobacter clade. Representatives of this clade are known to be metabolically versatile and involved in marine carbon-producing and biogeochemical processes. They form a physiologically heterogeneous group of Alphaproteobacteria and were mostly found in coastal or polar waters, especially in symbiosis with algae, in microbial mats, in sediments or together with invertebrates and vertebrates. Here we describe the features of L. arenae DSM 19593T, including novel aspects of its phenotype, together with the draft genome sequence and annotation. The 3,690,113 bp long genome consists of 17 scaffolds with 3,601 protein-coding and 56 RNA genes. This genome was sequenced as part of the activities of the Transregional Collaborative Research Centre 51 funded by the German Research Foundation (DFG).

Keywords

marine rod-shaped sea sand sediment motile strictly aerobic mesophile chemoorganotrophic halophilic virus-like structures carbon monoxide utilization sulfur oxidation Rhodobacteraceae Alphaproteobacteria Thalassobacter arenae

Introduction

Strain GA2-M15T (= DSM 19593 = KACC 12675) is the type strain of the species Litoreibacter arenae [1,2]. The genus Litoreibacter is a member of the highly abundant marine Roseobacter lineage, which plays an important role in the global carbon and sulfur cycles and thus for the climate on Earth. Phylogenetically, this alphaproteobacterial genus is related to the genera Jannaschia, Octadecabacter and Thalassobius [1]. Strain GA2-M15T was isolated from a sea-sand sample from the coast of Homi Cape, Pohang City, South Korea as Thalassobacter arenae [1], which was later on reclassified into Litoreibacter arenae [2]. The name for the genus Litoreibacter was constructed from litoreus (‘belonging to the seashore’) and bacter (‘a rod’) [3]. The species epithet arenae refers to the Neolatin adjective arenae, ‘of sand’. Current PubMed records do not indicate any follow-up research with strain GA2-M15T after the initial description of T. arenae [1] and its reclassification into L. arenae [2]. Here we present a summary classification and a set of features for L. arenae DSM 19593T, including novel aspects of its phenotype, together with the description of the genomic sequencing and annotation.

Classification and features of the organism

16S rRNA gene analysis

A representative genomic 16S rRNA gene sequence of L. arenae DSM 19593T was compared using NCBI BLAST [4,5] under default settings (e.g., considering only the high-scoring segment pairs (HSPs) from the best 250 hits) with the most recent release of the Greengenes database [6] and the relative frequencies of taxa and keywords (reduced to their stem [7]) were determined, weighted by BLAST scores. The most frequently occurring genera were Jannaschia (38.1%), Thalassobacter (15.4%), Octadecabacter (11.7%), Roseovarius (10.7%) and Roseobacter (10.2%) (28 hits in total). Regarding the three hits to sequences from other members of the genus, the average identity within HSPs was 96.0%, whereas the average coverage by HSPs was 98.7%. Among all other species, the one yielding the highest score was ‘Octadecabacter orientus’ (DQ167247), which corresponded to an identity of 99.2% and an HSP coverage of 99.6%. (Note that the Greengenes database uses the INSDC (= EMBL/NCBI/DDBJ) annotation, which is not an authoritative source for nomenclature or classification). The highest-scoring environmental sequence was FJ664800 (Greengenes short name ‘Quantitative dynamics cells plankton-fed microbial fuel cell clone plankton D11’), which showed an identity of 97.0% and an HSP coverage of 99.6%. The most frequently occurring keywords within the labels of all environmental samples which yielded hits were ‘lake’ (9.9%), ‘tin’ (9.8%), ‘xiaochaidan’ (9.4%), ‘microbi’ (2.6%) and ‘sea’ (2.5%) (222 hits in total). Environmental samples which yielded hits of a higher score than the highest scoring species were not found.

Figure 1 shows the phylogenetic neighborhood of L. arenae in a 16S rRNA sequence based tree. The sequence of the single 16S rRNA gene in the genome does not differ from the previously published 16S rDNA sequence (EU342372).
Figure 1.

Phylogenetic tree highlighting the position of L. arenae relative to the type strains of the type species of the other genera within the family Rhodobacteraceae. The tree was inferred from 1,330 aligned characters [8,9] of the 16S rRNA gene sequence under the maximum likelihood (ML) criterion [10]. Rooting was done initially using the midpoint method [11] and then checked for its agreement with the current classification (Table 1). The branches are scaled in terms of the expected number of substitutions per site. Numbers adjacent to the branches are support values from 650 ML bootstrap replicates [12] (left) and from 1,000 maximum-parsimony bootstrap replicates [13] (right) if larger than 60%. Lineages with type strain genome sequencing projects registered in GOLD [14] are labeled with one asterisk, those also listed as ‘Complete and Published’ with two asterisks [15].

Morphology and physiology

Cells of strain GA2-M15T are Gram-negative short rods (0.7-1.2 µm in width and 1.2–2.4 µm in length) and contain a polar flagellum for motility [1], [Figure 2]. Polyhydroxybutyrate is accumulated in the cells. Colonies are deep-brown, circular and contain clear margins. Cells are catalase and oxidase positive [1]. Growth occurs at 5–35 °C with an optimum at 30 °C. Cells were successfully grown on marine agar (MA), nutrient agar (NA, weak growth), salt tolerance agar (STA, containing 1% (w/v) tryptone, 0.3% (w/v) yeast extract and 1.5% (w/v) agar supplemented with salts) as well as on basal medium agar (BMA, recipe after [1]). No growth was observed on Reasoner’s 2A agar (R2A), trypticase soy agar (TSA) or MacConkey agar. The salinity range for growth is 0.85–8% NaCl (w/v), but the strain does not grow below 0.34% or at above 10% NaCl (w/v). The pH range for growth is pH 6–9 with an optimum at pH 7 [1].
Figure 2.

Micrograph of L. arenae DSM 19593T.

Cells hydrolyze aesculin and tyrosine weakly, but do not show any hydrolysis of alginic acid, casein, chitin, CM-cellulose, DNA, gelatin, pectin, starch or urea [1]. They assimilate citrate, D-fructose, D-galactose, D-glucose, L-glutamate, glycerol, β-hydroxybutyrate, D-mannitol, D-mannose, melibiose, propionate, pyruvate, L-serine, L-tyrosine and D-xylose, but not L-alanine, L-arabinose, L-aspartate, cellobiose, glycine, L-histidine, lactose, L-leucine, maltose, L-rhamnose, D-ribose, sucrose, L-threonine or trehalose. Cells are positive for β-galactosidase, esterase (C4), esterase lipase (C8), leucine arylamidase, naphthol-AS-BI-phosphohydrolase, α-glucosidase and β-glucosidase, but negative for indole production, arginine dihydrolase, alkaline phosphatase, lipase (C14), valine arylamidase, cystine arylamidase, trypsin, α-chymotrypsin, acid phosphatase, α-galactosidase, β-glucuronidase, N-acetyl-β-glucosaminidase, α-mannosidase and α-fucosidase [1].

The substrate utilization and resistance patterns of L. arenae DSM 19593T were also determined for this study using Generation-III microplates in an OmniLog phenotyping device (BIOLOG Inc., Hayward, CA, USA). The microplates were inoculated at 28°C with a cell suspension at a cell density of 95–96% turbidity and dye IF-A. Further additives were vitamin, micronutrient and sea salt solutions. The exported measurement data were further analyzed with the opm package for R [26,27], using its functionality for statistically estimating parameters from the respiration curves such as the maximum height, and automatically translating these values into negative, ambiguous, and positive reactions. The strain was studied in two independent biological replicates, and reactions with a different behavior between the two repetitions, if any, were regarded as ambiguous.

L. arenae DSM 19593T showed positive reactions for pH 6, 1% NaCl, 4% NaCl, D-galactose, 3-O-methyl-D-glucose, D-fucose, L-fucose, L-rhamnose, 1% sodium lactate, myo-inositol, rifamycin SV, L-aspartic acid, L-glutamic acid, L-histidine, L-serine, D-glucuronic acid, glucuronamide, quinic acid, L-lactic acid, citric acid, α-keto-glutaric acid, D-malic acid, L-malic acid, nalidixic acid, lithium chloride, acetic acid and sodium formate.

No reaction was found for dextrin, D-maltose, D-trehalose, D-cellobiose, β-gentiobiose, sucrose, D-turanose, stachyose, pH 5, D-raffinose, α-D-lactose, D-melibiose, β-methyl-D-galactoside, D-salicin, N-acetyl-D-glucosamine, N-acetyl-β-D-mannosamine, N-acetyl-D-galactosamine, N-acetyl-neuraminic acid, 8% NaCl, D-glucose, D-mannose, D-fructose, inosine, fusidic acid, D-serine, D-sorbitol, D-mannitol, D-arabitol, glycerol, D-glucose-6-phosphate, D-fructose-6-phosphate, D-aspartic acid, D-serine, troleandomycin, minocycline, gelatin, glycyl-L-proline, L-alanine, L-arginine, L-pyroglutamic acid, lincomycin, guanidine hydrochloride, niaproof, pectin, D-galacturonic acid, L-galactonic acid-γ-lactone, D-gluconic acid, mucic acid, D-saccharic acid, vancomycin, tetrazolium violet, tetrazolium blue, p-hydroxy-phenylacetic acid, methyl pyruvate, D-lactic acid methyl ester, bromo-succinic acid, potassium tellurite, tween 40, γ-amino-n-butyric acid, α-hydroxy-butyric acid, β-hydroxy-butyric acid, α-keto-butyric acid, acetoacetic acid, propionic acid, aztreonam, butyric acid and sodium bromate.

The measured utilization of carbon sources differs in some aspects from the one recorded in [1]. L-histidine and L-rhamnose were reported in [1] not to support bacterial growth, whereas in the Omnilog measurements both substrates yielded a positive reaction. This may be due to the higher sensitivity of respiratory measurements [28]. The utilization of propionate, D-fructose, D-glucose, D-mannose, D-mannitol, melibiose and glycerol reported by [1] could not be confirmed by the Omnilog measurements. Changes in the substrate-utilization pattern may arise from distinct cultivation conditions such as growth medium and temperature.

Chemotaxonomy

The principal cellular fatty acids of strain GA2-M15T are C18:1 ω7c (74.3%), C16:0 (10.4%), C18:1 ω7c 11-methyl (5.9%), C10:0 3-OH (3.7%) as well as an unknown fatty acid 11.799 (3.0%) [1]. In comparison to Thalassobacter stenotrophicus DSM 16310T [29,30], strain GA2-M15T reflected a higher content of C16:0 (1.1% vs 10.4%) [1]. The predominant polar lipids are diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidyl-choline [1].

Genome sequencing and annotation

Genome project history

The strain was first chosen for genome sequencing in the Genomic Encyclopedia of Bacteria and Archaea (GEBA) phase I project according the GEBA criteria [31,32], but then sequenced as part of the “Ecology, Physiology and Molecular Biology of the Roseobacter clade project: Towards a Systems Biology Understanding of a Globally Important Clade of Marine Bacteria” funded by the German Research Council (DFG). Project information is stored in the Genomes OnLine Database [33]. The Whole Genome Shotgun (WGS) sequence is deposited in Genbank and the Integrated Microbial Genomes database (IMG) [34]. A summary of the project information is shown in Table 2.
Table 1.

Classification and general features of L. arenae DSM 19593T according to the MIGS recommendations [16] published by the Genome Standards Consortium [17].

MIGS ID

Property

Term

Evidence code

 

Current classification

Domain Bacteria

TAS [18]

 

Phylum Proteobacteria

TAS [19]

 

Class Alphaproteobacteria

TAS [20,21]

 

Order Rhodobacterales

TAS [21,22]

 

Family Rhodobacteraceae

TAS [21,23]

 

Genus Litoreibacter

TAS [2,3]

 

Species Litoreibacter arenae

TAS [2]

MIGS-7

Subspecific genetic lineage (strain)

GA2-M15T

TAS [1]

MIGS-12

Reference for biomaterial

Kim et al. 2009

TAS [1]

 

Gram stain

negative

TAS [1]

 

Cell shape

rod-shaped

TAS [1]

 

Motility

motile

TAS [1]

 

Sporulation

non-sporulating

NAS

 

Temperature range

mesophile (5°C–35°C)

TAS [1]

 

Optimum temperature

30°C

TAS [1]

 

Salinity

halophilic, 0.85–8% NaCl (w/v)

TAS [1]

MIGS-22

Relationship to oxygen

strictly aerobic

TAS [1]

 

Carbon source

yeast extract, peptone

TAS [1]

MIGS-6

Habitat

sea sand, sediment, seawater

TAS [1]

MIGS-6.2

pH

6–9

TAS [1]

MIGS-15

Biotic relationship

free living

TAS [1]

 

Biosafety level

1

TAS [24]

MIGS-23.1

Isolation

sea sand

TAS [1]

MIGS-4

Geographic location

Coast of Homi Cape, Pohang City, South Korea

TAS [1]

MIGS-4.1

Latitude

36.085

NAS

MIGS-4.2

Longitude

129.556

NAS

MIGS-4.3

Depth

not reported

 

Evidence codes - TAS: Traceable Author Statement (i.e., a direct report exists in the literature); NAS: Non-traceable Author Statement (i.e., not directly observed for the living, isolated sample, but based on a generally accepted property for the species, or anecdotal evidence). Evidence codes are from the Gene Ontology project [25].

Table 2.

Genome sequencing project information

MIGS ID

Property

Term

MIGS-31

Finishing quality

Non-contiguous finished

MIGS-28

Libraries used

One Illumina PE library (520 bp insert size)

MIGS-29

Sequencing platforms

Illumina GA IIx, Illumina MiSeq

MIGS-31.2

Sequencing coverage

195 × Illumina

MIGS-30

Assemblers

velvet version 1.1.36, consed version 20.0

MIGS-32

Gene calling method

Prodigal

 

GOLD ID

Gi11991

 

NCBI project ID

178144

 

INSDC ID

AONI00000000

 

Genbank Date of Release

March 13, 2013

 

IMG Taxon OID

2518285519

MIGS-13

Source material identifier

DSM 19593

 

Project relevance

Tree of Life, biodiversity

Growth conditions and DNA isolation

A culture of DSM 19593T was grown aerobically in DSMZ medium 514 [35] at 28°C. Genomic DNA was isolated using Jetflex Genomic DNA Purification Kit (GENOMED 600100) following the standard protocol provided by the manufacturer but modified by an incubation time of 60 min, incubation on ice over night on a shaker, the use of additional 50 µl proteinase K, and the addition of 100 µl protein precipitation buffer. DNA is available from DSMZ through the DNA Bank Network [36].

Genome sequencing and assembly

The genome was sequenced using one Illumina PE library (Table 2). Illumina sequencing [37] was performed on a GA IIx platform with 150 cycles. The paired-end library contained 520 bp insert size. To correct sequencing errors and improve quality of the reads, clipping was performed using fastq-mcf [38] and quake [39]. After this step 4,717,610 reads with a median length of 124 bp were assembled using velvet [40]. The resulting draft genome consisted of 71 contigs organized in 45 scaffolds. The initial draft sequences were separated into artificial Sanger reads of 1,000 nt size plus 75 nt overlap. The number of gaps was reduced by manual editing in phred/phrap/consed version 20.0 [41]. The final assembly was composed of 17 contigs organized in 15 scaffolds. (The version deposited at Genbank contains two scaffolds less, which did not meet the requirements for the minimal contig length.) The additional fragments ‘thalar_Contig12.1’ and ‘thalar_Contig18_1.4’ can be found in the IMG database). The combined sequences provided a 195× coverage of the genome.

Genome annotation

Genes were identified using Prodigal [42] as part of the JGI genome annotation pipeline [43]. The predicted CDSs were translated and used to search the National Center for Biotechnology Information (NCBI) nonredundant database, UniProt, TIGR-Fam, Pfam, PRIAM, KEGG, COG, and InterPro databases. Identification of RNA genes were carried out by using HMMER 3.0rc1 [44] (rRNAs) and tRNAscan-SE 1.23 [45] (tRNAs). Other non-coding genes were predicted using INFERNAL 1.0.2 [46]. Additional gene prediction analysis and functional annotation was performed within the Integrated Microbial Genomes - Expert Review (IMG-ER) platform [34]. CRISPR elements were detected using CRT [47] and PILER-CR [48].

Genome properties

The genome statistics are provided in Table 3 and Figure 3. The genome consists of a 3.56 Mbp chromosome and a G+C content of 60% and a 140 kbp plasmid with a G+C content of 59%. Of the 3,657 genes predicted, 3,601 were protein-coding genes, and 56 RNAs. The majority of the protein-coding genes (81.8%) were assigned a putative function while the remaining ones were annotated as hypothetical proteins. The distribution of genes into COGs functional categories is presented in Table 4.
Figure 3.

Graphical map of the largest scaffold. From bottom to top: genes on forward strand (color by COG categories), genes on reverse strand (color by COG categories), RNA genes (tRNAs green, rRNAs red, other RNAs black), GC content, GC skew.

Table 3.

Genome Statistics

Attribute

Number

% of Total

Genome size (bp)

3,690,113

100.00

DNA coding region (bp)

3,376,611

91.50

DNA G+C content (bp)

2,222,524

60.23

Number of scaffolds

17

 

Extrachromosomal elements

1

 

Total genes

3,657

100.00

RNA genes

56

1.53

rRNA operons

1

 

tRNA genes

43

1.18

Protein-coding genes

3,601

98.47

Genes with function prediction (proteins)

2,990

81.76

Genes in paralog clusters

1,040

28.44

Genes assigned to COGs

2,873

78.56

Genes assigned Pfam domains

3,047

83.32

Genes with signal peptides

347

9.49

Genes with transmembrane helices

836

22.86

CRISPR repeats

0

 
Table 4.

Number of genes associated with the general COG functional categories

Code

Value

%age

Description

J

164

5.2

Translation, ribosomal structure and biogenesis

A

0

0.0

RNA processing and modification

K

197

6.3

Transcription

L

135

4.3

Replication, recombination and repair

B

3

0.1

Chromatin structure and dynamics

D

32

1.0

Cell cycle control, cell division, chromosome partitioning

Y

0

0.0

Nuclear structure

V

46

1.5

Defense mechanisms

T

97

3.1

Signal transduction mechanisms

M

176

5.6

Cell wall/membrane/envelope biogenesis

N

32

1.0

Cell motility

Z

2

0.1

Cytoskeleton

W

0

0.0

Extracellular structures

U

73

2.3

Intracellular trafficking and secretion, and vesicular transport

O

121

3.8

Posttranslational modification, protein turnover, chaperones

C

195

6.2

Energy production and conversion

G

200

6.4

Carbohydrate transport and metabolism

E

358

11.4

Amino acid transport and metabolism

F

82

2.6

Nucleotide transport and metabolism

H

138

4.4

Coenzyme transport and metabolism

I

146

4.6

Lipid transport and metabolism

P

141

4.5

Inorganic ion transport and metabolism

Q

99

3.1

Secondary metabolites biosynthesis, transport and catabolism

R

410

13.0

General function prediction only

S

303

9.6

Function unknown

-

784

21.4

Not in COGs

Insights into the genome

The genome comprises a single extrachromosomal element (with not yet validated circularity), ‘thalar_Contig204.17’, 139.9 kbp in size containing 130 protein-coding genes including a large RTX-toxin gene and a F0F1-type ATPase operon. It contains the typical replication modules. Its replication system is of the ABC-9 type with the compatibility group RepC-9 [49]. This type of repABC operon was found in two representatives of the genera Octadecabacter and Roseobacter, respectively, as well as in Dinoroseobacter shibae [49]. The presence of the replication-initiation gene DnaA (Thalar_03034) reveals the chromosomal origin of largest, 1.091 Mbp long scaffold ‘thalar_Contig148.14’, but it harbors neither a plasmid stability module nor a type IV secretion system.

Genome analysis of strain DSM 19593T revealed the presence of genes encoding proteins associated to carbon monoxide utilization (thalar_00241, thalar_00242, thalar_02265, thalar_03324, thalar_03325, thalar_03395, thalar_03397) as well as genes forming a putative operon, which are involved in the oxidation of sulfur (thalar_01786 to_01792) indicating the oxidation of sulfur to produce energy. Additional gene sequences of interest encode a homogentisate 1,2-dioxygenase (thalar_03573), several haloacid dehalogenase superfamily proteins (thalar_00489, thalar_00580, thalar_01120, thalar_01943, thalar_02401) and a 2-haloalkanoic acid dehalogenase type II (thalar_00287). The presence of such genes could indicate a respiratory degradation of recalcitrant compounds by strain DSM 19593T in its ecological niche.

Further genes encoding a N-acyl-L-homoserine lactone synthetase (thalar_00160) and a response regulator (thalar_00161) associated to quorum sensing were observed [5053]. Genome analysis of strain DSM 19593T also revealed the presence of genes encoding a bacteriophage associated genes (e.g., thalar_00003 to 00007). A gene encoding a sensor of blue light using FAD (BLUF, thalar_02670) was also detected, indicating possible blue-light dependent signal transduction.

Declarations

Acknowledgements

The authors gratefully acknowledge the assistance of Iljana Schröder for technical assistance and Evelyne-Marie Brambilla for DNA extraction and quality control (both at the DSMZ). This study was supported by the German Research Foundation (DFG) Transregio-SFB 51.

Authors’ Affiliations

(1)
UMR 7621, Observatoire Océanologique, UPMC Université Paris 6
(2)
UMR 7621, LOMIC, Observatoire Océanologique, CNRS
(3)
Leibniz Institute, DSMZ - German Collection of Microorganisms and Cell Cultures
(4)
DOE Joint Genome Institute

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