Skip to content


Standards in Genomic Sciences

Open Access

High-quality permanent draft genome sequence of Rhizobium leguminosarum bv. viciae strain GB30; an effective microsymbiont of Pisum sativum growing in Poland

  • Andrzej Mazur1,
  • Sofie E. De Meyer2,
  • Rui Tian2,
  • Jerzy Wielbo1,
  • Kamil Zebracki1,
  • Rekha Seshadri3,
  • TBK Reddy3,
  • Victor Markowitz4,
  • Natalia N. Ivanova3,
  • Amrita Pati3,
  • Tanja Woyke3,
  • Nikos C. Kyrpides3, 5 and
  • Wayne Reeve2Email author
Standards in Genomic Sciences201510:36

Received: 18 March 2015

Accepted: 5 June 2015

Published: 16 July 2015


Rhizobium leguminosarum bv. viciae GB30 is an aerobic, motile, Gram-negative, non-spore-forming rod that can exist as a soil saprophyte or as a legume microsymbiont of Pisum sativum. GB30 was isolated in Poland from a nodule recovered from the roots of Pisum sativum growing at Janow. GB30 is also an effective microsymbiont of the annual forage legumes vetch and pea. Here we describe the features of R. leguminosarum bv. viciae strain GB30, together with sequence and annotation. The 7,468,464 bp high-quality permanent draft genome is arranged in 78 scaffolds of 78 contigs containing 7,227 protein-coding genes and 75 RNA-only encoding genes, and is part of the GEBA-RNB project proposal.


Root-nodule bacteriaNitrogen fixationRhizobiaAlphaproteobacteriaGEBA-RNB


The most efficient biological nitrogen fixation occurs when bacterial microsymbionts (rhizobia) form an effective symbiotic association with legume host plants. Legumes can develop these interactions with many different species of rhizobia belonging mainly to the Alphaproteobacteria , including Azorhizobium , Allorhizobium , Bradyrhizobium , Ensifer , Mesorhizobium and Rhizobium [1, 2]. The genus Rhizobium contains at the time of writing 71 species, and within a species there may be distinct symbiovars [3].

Within the species Rhizobium leguminosarum , there are three distinct symbiovars [4, 5] including bv. phaseoli that forms nodules with Phaseolus vulgaris , bv. trifolii that forms nodules with clover ( Trifolium ) and bv. viciae that forms nodules on vetch, pea and lentil ( Vicia , Lathyrus , Pisum and Lens ). In R. leguminosarum the nod genes that define these distinct host specificities are mostly located on the symbiotic plasmid, which has generically been designated pSym. The genomes of R. leguminosarum strains are usually large and complex containing, in addition to pSym, a chromosomal replicon and extra-chromosomal low-copy-number replicons characterized by the presence of repABC replication systems [68]. Recent studies have revealed that substantial divergence can occur in this genome organization and in the metabolic versatility of R. leguminosarum isolates [5, 912]. Kumar et al. [5] demonstrated that the diversity of R. leguminosarum within a local population of nodule isolates was 10 times higher than that found for Ensifer medicae . It was noted that the abundance of a particular genotype within the population can vary significantly and adaptation to the edaphic environment is a sought after trait particularly for the development of inoculants [13, 14].

R. leguminosarum bv. viciae GB30 was isolated as the most abundant nodule inhabitant (>42 %) of Pisum sativum cv. Ramrod plants cultivated at a field site in Janow, Poland [10]. In contrast to other abundant isolates, GB30 formed nodules and fixed nitrogen with both P. sativum and Vicia villosa (cv. Wista). Preliminary investigation into the genome architecture using Eckhardt analysis has revealed that GB30 contained a multipartite genome consisting of six replicons with one chromosome and five plasmids [10]. The genome of this strain could therefore provide important insights into the mechanisms required by effective R. leguminosarum microsymbionts to adapt to a particular edaphic environment. Here, we present a set of general features for Rhizobium leguminosarum bv. viciae GB30 together with the description of the complete genome sequence and annotation.

Organism information

Classification and features

R. leguminosarum bv. viciae strain GB30 is a motile, Gram-negative rod in the order Rhizobiales of the class Alphaproteobacteria . The rod-shaped form varies in size with dimensions of 0.8-1 μm in width and 2.3-2.5 μm in length (Fig. 1 Left and Center). It is fast growing, forming colonies within 3–4 days when grown on half strength Lupin Agar (½LA) [15] at 28 °C. Colonies on ½LA are white-opaque, slightly domed and moderately mucoid with smooth margins (Fig. 1 Right).
Fig. 1

Images of Rhizobium leguminosarum bv. viciae strain GB30 using scanning (Left) and transmission (Center) electron microscopy and the appearance of colony morphology on ½LA solid media (Right)

Figure 2 shows the phylogenetic relationship of Rhizobium leguminosarum bv. viciae GB30 in a 16S rRNA gene sequence based tree. This strain is phylogenetically most related to Rhizobium laguerreae FB206T and Rhizobium gallicum R602spT based on the 16S rRNA gene alignment with sequence identities of 100 %, as determined using the EzTaxon-e server [16]. Rhizobium laguerreae FB206T was isolated from effective Vicia faba root nodules in Tunisia [17], whereas Rhizobium gallicum R602spT was isolated from effective Phaseolus vulgaris root nodules in France [18]. Sequence similarity was also investigated with strains from the GEBA-RNB project [12] and GB30 was found to be closely related to R. leguminosarum bv. trifolii WSM1689 with 100 % 16S rRNA gene sequence identity. R. leguminosarum bv. trifolii WSM1689 is a highly effective microsymbiont of the perennial clover Trifolium uniflorum and has been shown to have a remarkable narrow host range [19]. Minimum Information about the Genome Sequence (MIGS) is provided in Table 1 and Additional file 1: Table S1.
Fig. 2

Phylogenetic tree highlighting the position of Rhizobium leguminosarum bv. viciae GB30 (shown in blue print) relative to other type and non-type strains in the Rhizobium genus using a 901 bp internal region of the 16S rRNA gene. Bradyrhizobium elkanii ATCC 49852T was used as outgroup. All sites were informative and there were no gap-containing sites. Phylogenetic analyses were performed using MEGA, version 5.05 [36]. The tree was built using the maximum likelihood method with the General Time Reversible model. Bootstrap analysis with 500 replicates was performed to assess the support of the clusters. Type strains are indicated with a superscript T. Strains with a genome sequencing project registered in GOLD [20] are shown in bold and have the GOLD ID mentioned after the strain number, otherwise the NCBI accession number has been provided. Finished genomes are designated with an asterisk

Table 1

Classification and general features of Rhizobium leguminosarum bv. viciae strain GB30 in accordance with the MIGS recommendations [37] published by the Genome Standards Consortium [38].




Evidence code


Domain Bacteria

TAS [39]


Phylum Proteobacteria

TAS [40, 41]


Class Alphaproteobacteria

TAS [42, 43]



Order Rhizobiales

TAS [44]


Family Rhizobiaceae

TAS [45]


Genus Rhizobium

TAS [46]


Species Rhizobium leguminosarum

TAS [4749]


Gram stain




Cell shape












Temperature range




Optimum temperature

28 °C

TAS [9]


pH range; Optimum

Not reported


Carbon source

Not reported




Soil, root nodule, on host

TAS [9]






Oxygen requirement


TAS [49]


Biotic relationship

Free living, symbiotic

TAS [10]




TAS [50]


Geographic location

Janow, near Lublin, eastern Poland

TAS [10]


Sample collection

Between May and June, 2008

TAS [10]




TAS [10]




TAS [10]



185 m


Evidence codes – IDA: Inferred from Direct Assay; TAS: Traceable Author Statement (i.e., a direct report exists in the literature); NAS: Non-traceable Author Statement (i.e., not directly observed for the living, isolated sample, but based on a generally accepted property for the species, or anecdotal evidence). These evidence codes are from the Gene Ontology project [51].


R. leguminosarum bv. viciae strain GB30 was obtained from pea nodules (P. sativum cv. Ramrod) growing in sandy loam (N:P:K 0.157:0.014:0.013 %) in Janow near Lublin (Poland). The soil contained a relatively high number of R. leguminosarum bv. viciae, bv. trifolii and bv. phaseoli cells i.e., 9.2 × 103, 4.2 ÷ 103 and 1.5 × 103 bacteria/g of soil, respectively, as determined by the most probable number (MPN) method [10]. Plants were grown on 1 m2 plot for six weeks between May and June, 2008. Five randomly chosen pea plants growing in each other’s vicinity were harvested; the nodules were collected, surface-sterilized and the microsymbionts isolated [10]. One of the most abundant isolates, GB30, formed nodules (Nod+) and fixed N2 (Fix+) with P. sativum and Vicia villosa (cv. Wista) increasing the wet mass weight by 54 and 38 %, respectively. Plants inoculated with GB30 also showed a 2.6 fold increase in nodule number and a 2.2 fold increase in seed pod number.

Genome sequencing and annotation information

Genome project history

This organism was selected for sequencing on the basis of its environmental and agricultural relevance to issues in global carbon cycling, alternative energy production, and biogeochemical importance, and is part of the Genomic Encyclopedia of Bacteria and Archaea, The Root Nodulating Bacteria chapter (GEBA-RNB) project at the U.S. Department of Energy, Joint Genome Institute [12]. The genome project is deposited in the Genomes OnLine Database [20] and the high-quality permanent draft genome sequence in IMG [21]. Sequencing, finishing and annotation were performed by the JGI using state of the art sequencing technology [22]. A summary of the project information is shown in Table 2.
Table 2

Genome sequencing project information for Rhizobium leguminosarum bv. viciae strain GB30





Finishing quality

High-quality permanent draft


Libraries used

Illumina Std PE


Sequencing platforms

Illumina Hiseq 2000


Fold coverage

121.9 x Illumina



Velvet version 1.1.04; ALLPATHS v. r41043


Gene calling methods

Prodigal 1.4


Locus Tag



GenBank ID



GenBank Date of Release

July 9, 2013



Gp0009658 [52]





Source Material Identifier



Project relevance

Symbiotic N2 fixation, agriculture

Growth conditions and genomic DNA preparation

R. leguminosarum bv. viciae strain GB30 was grown to mid logarithmic phase in TY rich media [23] on a gyratory shaker at 28 °C. DNA was isolated from 60 mL of cells using a CTAB (Cetyl trimethyl ammonium bromide) bacterial genomic DNA isolation method [24].

Genome sequencing and assembly

The draft genome of Rhizobium leguminosarum bv. viciae GB30 was generated at the DOE Joint Genome Institute [22]. An Illumina Std shotgun library was constructed and sequenced using the Illumina HiSeq 2000 platform which generated 25,943,396 reads totaling 3,891.5 Mbp. All general aspects of library construction and sequencing performed at the JGI can be found at the JGI web site [25]. All raw Illumina sequence data was passed through DUK, a filtering program developed at JGI, which removes known Illumina sequencing and library preparation artefacts (Mingkun L, Copeland A, Han J. unpublished). Following steps were then performed for assembly: (1) filtered Illumina reads were assembled using Velvet version 1.1.04 [26] (2) 1–3 Kbp simulated paired end reads were created from Velvet contigs using wgsim [27] (3) Illumina reads were assembled with simulated read pairs using Allpaths–LG (version r41043) [28]. Parameters for assembly steps were: 1) Velvet (velveth: 63 –shortPaired and velvetg: −very_clean yes –export-Filtered yes –min_contig_lgth 500 –scaffolding no –cov_cutoff 10) 2) wgsim (−e 0 –1 100 –2 100 –r 0 –R 0 –X 0) 3) Allpaths–LG (PrepareAllpathsInputs: PHRED_64 = 1 PLOIDY = 1 FRAG_COVERAGE = 125 JUMP_COVERAGE = 25 LONG_JUMP_COV = 50, RunAllpathsLG: THREADS = 8 RUN = std_shredpairs TARGETS = standard VAPI_WARN_ONLY = True OVERWRITE = True). The final draft assembly contained 78 contigs in 78 scaffolds. The total size of the genome is 7.5 Mbp and the final assembly is based on 910.4 Mbp of Illumina data, which provides an average of 121.9× coverage.

Genome annotation

Genes were identified using Prodigal [29], as part of the DOE-JGI genome annotation pipeline [30, 31]. The predicted CDSs were translated and used to search the National Centre for Biotechnology Information (NCBI) non-redundant database, UniProt, TIGRFam, Pfam, KEGG, COG, and InterPro databases. The tRNAScanSE tool [32] was used to find tRNA genes, whereas ribosomal RNA genes were found by searches against models of the ribosomal RNA genes built from SILVA [33]. Other non–coding RNAs such as the RNA components of the protein secretion complex and the RNase P were identified by searching the genome for the corresponding Rfam profiles using INFERNAL [34]. Additional gene prediction analysis and manual functional annotation was performed within the Integrated Microbial Genomes-Expert Review (IMG-ER) system [35] developed by the Joint Genome Institute, Walnut Creek, CA, USA.

Genome Properties

The genome is 7,468,464 nucleotides with 60.81 % GC content (Table 3) and comprised of 78 scaffolds of 78 contigs. From a total of 7,302 genes, 7,227 were protein encoding and 75 RNA only encoding genes. The majority of genes (79.57 %) were assigned a putative function whilst the remaining genes were annotated as hypothetical. The distribution of genes into COGs functional categories is presented in Table 4.
Table 3

Genome Statistics for Rhizobium leguminosarum bv. viciae strain GB30



% of Total

Genome size (bp)



DNA coding (bp)



DNA G + C (bp)



DNA scaffolds



Total genes



Protein coding genes



RNA genes



Pseudo genes



Genes in internal clusters



Genes with function prediction



Genes assigned to COGs



Genes with Pfam domains



Genes with signal peptides



Genes with transmembrane proteins



CRISPR repeats


Table 4

Number of genes associated with the general COG functional categories.



% age





Translation, ribosomal structure and biogenesis




RNA processing and modification








Replication, recombination and repair




Chromatin structure and dynamics




Cell cycle control, Cell division, chromosome partitioning




Defense mechanisms




Signal transduction mechanisms




Cell wall/membrane/envelope biogenesis




Cell motility




Intracellular trafficking, secretion, and vesicular transport




Posttranslational modification, protein turnover, chaperones




Energy production and conversion




Carbohydrate transport and metabolism




Amino acid transport and metabolism




Nucleotide transport and metabolism




Coenzyme transport and metabolism




Lipid transport and metabolism




Inorganic ion transport and metabolism




Secondary metabolite biosynthesis, transport and catabolism




General function prediction only




Function unknown




Not in COGS

The total is based on the total number of protein coding genes in the genome.


Rhizobium leguminosarum bv. viciae GB30 belongs to a group of Alpha-rhizobia strains isolated from Pisum sativum in Poland. Strain GB30 is part of the GEBA-RNB project that sequenced 24 R. leguminosarum strains and 12 R. leguminosarum bv. viciae strains [12]. Phylogenetic analysis revealed that GB30 is most closely related to Rhizobium leguminosarum bv. trifolii CB782 and WSM1689, both part of the GEBA-RNB project [12]. Full genome comparison of GB30 and WSM1689 [19] revealed that GB30 has the largest genome (7.4 Mbp), with the highest COG count (5,182), the lowest Pfam % (82.51) and the lowest TIGRfam % (22.13 %). The genome attributes of R. leguminosarum bv. viciae GB30, in conjunction with the other R. leguminosarum genomes, will be important for on-going comparative and functional analyses of the plant microbe interactions required for the successful establishment of agricultural crops.



Genomic Encyclopedia of Bacteria and Archaea – Root Nodule Bacteria


Joint Genome Institute


Trypton Yeast


Cetyl trimethyl ammonium bromide


Western Australian Soil Microbiology


Most probable number


Integrated Microbial Genomes-Expert Review


National Centre for Biotechnology Information



This work was performed under the auspices of the US Department of Energy’s Office of Science, Biological and Environmental Research Program, and by the University of California, Lawrence Berkeley National Laboratory under contract No. DE-AC02-05CH11231, Lawrence Livermore National Laboratory under Contract No. DE-AC52-07NA27344, and Los Alamos National Laboratory under contract No. DE-AC02-06NA25396.

Authors’ Affiliations

Department of Genetics and Microbiology, Maria Curie Sklodowska University, Lublin, Poland
Centre for Rhizobium Studies, Murdoch University, Murdoch, Australia
DOE Joint Genome Institute, Walnut Creek, USA
Biological Data Management and Technology Center, Lawrence Berkeley National Laboratory, Berkeley, USA
Department of Biological Sciences, King Abdulaziz University, Jeddah, Saudi Arabia


  1. Franche C, Lindstrom K, Elmerich C. Nitrogen-fixing bacteria associated with leguminous and non-leguminous plants. Plant Soil. 2009;321:35–59.View ArticleGoogle Scholar
  2. Masson-Boivin C, Giraud E, Perret X, Batut J. Establishing nitrogen-fixing symbiosis with legumes: how many rhizobium recipes? Trends Microbiol. 2009;17:458–66.View ArticlePubMedGoogle Scholar
  3. List of prokaryotic names with standing in nomenclature []
  4. Rogel MA, Ormeño-Orrillo E, Martinez Romero E. Symbiovars in rhizobia reflect bacterial adaptation to legumes. Syst Appl Microbiol. 2011;34:96–104.View ArticlePubMedGoogle Scholar
  5. Kumar N, Lad G, Giuntini E, Kaye ME, Udomwong P, Shamsani NJ, Young JPW, Bailly X: Bacterial genospecies that are not ecologically coherent: population genomics of Rhizobium leguminosarum. Open biol 2015, 5.Google Scholar
  6. Young JPW, Crossman LC, Johnston AWB, Thomson NR, Ghazoui ZF, Hull KH, Wexler M, Curson ARJ, Todd JD, Poole PS, Mauchline TH, East AK, Quail MA, Churcher C, Arrowsmith C, Cherevach I, Chillingworth T, Clarke K, Cronin A, Davis P, Fraser A, Hance Z, Hauser H, Jagels K, Moule S, Mungall K, Norbertczak H, Rabbinowitsch E, Sanders M, Simmonds M, et al.: The genome of Rhizobium leguminosarum has recognizable core and accessory components. Genome Biol 2006, 7Google Scholar
  7. Reeve W, O'Hara G, Chain P, Ardley J, Brau L, Nandesena K, et al. Complete genome sequence of Rhizobium leguminosarum bv. trifolii strain WSM1325, an effective microsymbiont of annual Mediterranean clovers. Stand Genomic Sci. 2010;2:347–56.View ArticlePubMedPubMed CentralGoogle Scholar
  8. Reeve W, O’Hara G, Chain P, Ardley J, Brau L, Nandesena K, et al. Complete genome sequence of Rhizobium leguminosarum bv trifolii strain WSM2304, an effective microsymbiont of the South American clover Trifolium polymorphum. Stand Genomic Sci. 2010;2:66–76.View ArticlePubMedPubMed CentralGoogle Scholar
  9. Wielbo J, Marek-Kozaczuk M, Mazur A, Kubik-Komar A, Skorupska A. Genetic and metabolic divergence within a Rhizobium leguminosarum bv. trifolii population recovered from clover nodules. Appl Environ Microbiol. 2010;76:4593–600.View ArticlePubMedPubMed CentralGoogle Scholar
  10. Wielbo J, Marek-Kozaczuk M, Mazur A, Kubik-Komar A, Skorupska A. The structure and metabolic diversity of population of pea microsymbionts isolated from root nodules. BMRJ. 2011;1:55–69.View ArticleGoogle Scholar
  11. Mazur A, Stasiak G, Wielbo J, Kubik-Komar A, Marek-Kozaczuk M, Skorupska A: Intragenomic diversity of Rhizobium leguminosarum bv. trifolii clover nodule isolates. BMC Microbiol 2011, 11.Google Scholar
  12. Reeve W, Ardley J, Tian R, Eshragi L, Yoon J, Ngamwisetkun P, et al. A genomic encyclopedia of the root nodule bacteria: Assessing genetic diversity through a systematic biogeographic survey. Stand Genomic Sci. 2015;10:14.View ArticlePubMedPubMed CentralGoogle Scholar
  13. Howieson J, Ballard R. Optimising the legume symbiosis in stressful and competitive environments within southern Australia - some contemporary thoughts. Soil Biol Biochem. 2004;36:1261–73.View ArticleGoogle Scholar
  14. Howieson JG, Yates RJ, Foster K, Real D, Besier B. Prospects for the future use of legumes. In: Dilworth MJ, James EK, Sprent JI, Newton WE, editors. Leguminous Nitrogen-Fixing Symbioses. London, UK: Elsevier; 2008. p. 363–94.Google Scholar
  15. Howieson JG, Ewing MA, D’antuono MF. Selection for acid tolerance in Rhizobium meliloti. Plant Soil. 1988;105:179–88.View ArticleGoogle Scholar
  16. Kim O-S, Cho Y-J, Lee K, Yoon S-H, Kim M, Na H, et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol. 2012;62:716–21.View ArticlePubMedGoogle Scholar
  17. Saïdi S, Ramírez-Bahena M-H, Santillana N, Zúñiga D, Álvarez-Martínez E, Peix A, et al. Rhizobium laguerreae sp. nov. nodulates Vicia faba on several continents. Int J Syst Evol Microbiol. 2014;64:242–7.View ArticlePubMedGoogle Scholar
  18. Amarger N, Macheret V, Laguerre G. Rhizobium gallicum sp. nov. and Rhizobium giardinii sp. nov., from Phaseolus vulgaris nodules. Int J Syst Bacteriol. 1997;47:996–1006.View ArticlePubMedGoogle Scholar
  19. Terpolilli J, Rui T, Yates R, Howieson J, Poole P, Munk C, et al. Genome sequence of Rhizobium leguminosarum bv trifolii strain WSM1689, the microsymbiont of the one flowered clover Trifolium uniflorum. Stand Genomic Sci. 2014;9:527–39.View ArticlePubMedPubMed CentralGoogle Scholar
  20. Pagani I, Liolios K, Jansson J, Chen IM, Smirnova T, Nosrat B, et al. The Genomes OnLine Database (GOLD) v. 4: status of genomic and metagenomic projects and their associated metadata. Nucleic Acids Res. 2012;40:D571–579.View ArticlePubMedPubMed CentralGoogle Scholar
  21. Markowitz VM, Chen I-MA, Palaniappan K, Chu K, Szeto E, Pillay M, et al. IMG 4 version of the integrated microbial genomes comparative analysis system. Nucleic Acids Res. 2014;42:D560–7.View ArticlePubMedPubMed CentralGoogle Scholar
  22. Mavromatis K, Land ML, Brettin TS, Quest DJ, Copeland A, Clum A, et al. The fast changing landscape of sequencing technologies and their impact on microbial genome assemblies and annotation. PLoS ONE. 2012;7, e48837.View ArticlePubMedPubMed CentralGoogle Scholar
  23. Beringer JE. R factor transfer in Rhizobium leguminosarum. J Gen Microbiol. 1974;84:188–98.PubMedGoogle Scholar
  24. CTAB DNA extraction protocol []
  25. JGI Website []
  26. Zerbino D, Birney E. Velvet: algorithms for de novo short read assembly using de Bruijn graphs. Genome Res. 2008;18:821–9.View ArticlePubMedPubMed CentralGoogle Scholar
  27. wgsim []
  28. Gnerre S, MacCallum I, Przybylski D, Ribeiro FJ, Burton JN, Walker BJ, et al. High-quality draft assemblies of mammalian genomes from massively parallel sequence data. PNAS USA. 2011;108:1513–8.View ArticlePubMedPubMed CentralGoogle Scholar
  29. Hyatt D, Chen GL, Locascio PF, Land ML, Larimer FW, Hauser LJ. Prodigal: prokaryotic gene recognition and translation initiation site identification. BMC Bioinformatics. 2010;11:119.View ArticlePubMedPubMed CentralGoogle Scholar
  30. Mavromatis K, Ivanova NN, Chen IM, Szeto E, Markowitz VM, Kyrpides NC. The DOE-JGI Standard Operating Procedure for the annotations of microbial genomes. Standa Genomic Sci. 2009;1:63–7.View ArticleGoogle Scholar
  31. Chen IM, Markowitz VM, Chu K, Anderson I, Mavromatis K, Kyrpides NC, et al. Improving microbial genome annotations in an integrated database context. PLoS ONE. 2013;8, e54859.View ArticlePubMedPubMed CentralGoogle Scholar
  32. Lowe TM, Eddy SR. tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence. Nucleic Acids Res. 1997;25:955–64.View ArticlePubMedPubMed CentralGoogle Scholar
  33. Pruesse E, Quast C, Knittel K, Fuchs BM, Ludwig W, Peplies J, et al. SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB. Nucleic Acids Res. 2007;35:7188–96.View ArticlePubMedPubMed CentralGoogle Scholar
  34. INFERNAL. Inference of RNA alignments []
  35. Markowitz VM, Mavromatis K, Ivanova NN, Chen IM, Chu K, Kyrpides NC. IMG ER: a system for microbial genome annotation expert review and curation. Bioinformatics. 2009;25:2271–8.View ArticlePubMedGoogle Scholar
  36. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: Molecular Evolutionary Genetics Analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 2011;28:2731–9.View ArticlePubMedPubMed CentralGoogle Scholar
  37. Field D, Garrity G, Gray T, Morrison N, Selengut J, Sterk P, et al. Towards a richer description of our complete collection of genomes and metagenomes “Minimum Information about a Genome Sequence” (MIGS) specification. Nat Biotechnol. 2008;26:541–7.View ArticlePubMedPubMed CentralGoogle Scholar
  38. Field D, Amaral-Zettler L, Cochrane G, Cole JR, Dawyndt P, Garrity GM, et al. The Genomic Standards Consortium. PLoS Biol. 2011;9:e1001088.View ArticlePubMedPubMed CentralGoogle Scholar
  39. Woese CR, Kandler O, Wheelis ML. Towards a natural system of organisms: proposal for the domains Archaea, Bacteria, and Eucarya. PNAS USA. 1990;87:4576–9.Google Scholar
  40. Chen WX, Wang ET, Kuykendall LD. The Proteobacteria. New York: Springer - Verlag; 2005.Google Scholar
  41. Validation of publication of new names and new combinations previously effectively published outside the IJSEM. Int J Syst Evol Microbiol 2005, 55:2235–2238.Google Scholar
  42. List of new names and new combinations previously effectively, but not validly, published. Int J Syst Evol Microbiol 2006, 56:1–6.Google Scholar
  43. Garrity GM, Bell JA, Lilburn T. Class I. Alphaproteobacteria class. In: Garrity GM, Brenner DJ, Kreig NR, Staley JT, editors. Bergey’s Manual of Systematic Bacteriology. Secondth ed. New York: Springer - Verlag; 2005.Google Scholar
  44. Kuykendall LD. Order VI. Rhizobiales ord. nov. In: Garrity GM, Brenner DJ, Kreig NR, Staley JT, editors. Bergey’s Manual of Systematic Bacteriology. Secondth ed. New York: Springer - Verlag; 2005. p. 324.Google Scholar
  45. Kuykendall LD. Family I. Rhizobiaceae In Bergey’s Manual of Systematic Bacteriology. Edited by Garrity GM, Brenner DJ, Krieg NR, Staley JT. New York: Springer - Verlag; 2005.Google Scholar
  46. Kuykendall LD, Young JM, Martínez-Romero E, Kerr A, Sawada H. Genus I. Rhizobium. In Bergey’s Manual of Systematic Bacteriology. Volume 2. Second edition. Edited by Garrity GM, Brenner DJ, Krieg NR, Staley JT. New York: Springer - Verlag; 2005.Google Scholar
  47. Skerman V, McGowan V, Sneath P. Approved lists of bacterial names. Int J Syst Evol Microbiol. 1980;30:225–420.View ArticleGoogle Scholar
  48. Young JM, Kuykendall LD, Martinez-Romero E, Kerr A, Sawada H. A revision of Rhizobium Frank 1889, with an emended description of the genus, and the inclusion of all species of Agrobacterium Conn 1942 and Allorhizobium undicola de Lajudie et al. 1998 as new combinations: Rhizobium radiobacter, R-rhizogenes, R-rubi, R-undicola and R-vitis. Int J Syst Evol Microbiol. 2001;51:89–103.View ArticlePubMedGoogle Scholar
  49. Ramirez-Bahena MH, Garcia-Fraile P, Peix A, Valverde A, Rivas R, Igual JM, et al. Revision of the taxonomic status of the species Rhizobium leguminosarum (Frank 1879) Frank 1889(AL), Rhizobium phaseoli Dangeard 1926(AL) and Rhizobium trifolii Dangeard 1926AL. R-trifolii is a later synonym of R-leguminosarum. Reclassification of the strain R-leguminosarum DSM 30132 (= NCIMB 11478) as Rhizobium pisi sp nov. Int J Syst Evol Microbiol. 2008;58:2484–90.View ArticlePubMedGoogle Scholar
  50. Biological Agents: Technical rules for biological agents. TRBA:466.Google Scholar
  51. Ashburner M, Ball CA, Blake JA, Botstein D, Butler H, Cherry JM, et al. Gene ontology: tool for the unification of biology. The Gene Ontology Consortium Nat Genet. 2000;25:25–9.PubMedGoogle Scholar
  52. GOLD ID Rhizobium leguminosarum bv. viciae GB30 []


© Mazur et al. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.