Figure 1.
Phylogenetic tree highlighting the position of Lysinibacillus sphaericus OT4b.31 relative to the available type strains and other non-assigned species within the families Alicyclobacillaceae and Bacillaceae. Alicyclobacillus cycloheptanicus was designated as the outgroup species for the analyses. Right brackets encompass each homology group (I–VII) according to Nakamura’s benchmarks [21]. Nucleotide sequences obtained from GenBank and used in the phylogenetic analyses were as follows: Alicyclobacillus cycloheptanicus 1457 (X51928), Geobacillus stearothermophilus 10 (X57309), Bacillus subtilis 168T (X60646), Bacillus licheniformis DSM 13T (X68416), Bacillus megaterium IAM 13418T (D16273), Bacillus sp. BD-87 (AF169520), Bacillus sp. BD-99 (AF169525), Bacillus sp. NRS-1691 (AF169531), Bacillus sp. NRS-1693 (AF169533), Solibacillus silvestris StLB046 (NR_074954), Lysinibacillus massiliensis 4400831 (NR_043092), Bacillus sp. NRS-250 (AF169536), Bacillus sp. B-1876 (AF169494), Bacillus sp. NRS-1198 (AF169528), Bacillus sp. B-4297 (AF169507), Bacillus sp. NRS-111 (AF169526), Lysinibacillus sphaericus OT4b.31 (AQPX00000042.1:91-1546), Bacillus sp. B-183 (AF169493), Lysinibacillus sphaericus B-23268T (AF169495), Lysinibacillus sphaericus JG-A12 (AM292655), Bacillus sp. B-14905 (AF169491), Lysinibacillus sphaericus ZC1 (NZ_ADJR01000054.1:1-1487), Lysinibacillus sphaericus C3-41 (NC_010382.1:16887-18287), Bacillus sp. B-14865 (AF169490), Lysinibacillus sphaericus 2362 (L14011), Lysinibacillus fusiformis ATCC-7055 (AJ310083), Bacillus sp. B-14957 (AF169492) and Bacillus sp. B-23269 (AF169496). The branches are scaled in terms of the expected number of substitutions per site. Numbers adjacent to the branches represent percentage bootstrap values based on 1,000 replicates. Lineages with type strain genome sequencing projects registered in GOLD [22] are labeled with one asterisk, those also listed as ‘Complete and Published’ with two asterisks.