Figure 1.
![Figure 1.](http://media.springernature.com/full/springer-static/image/art%3A10.4056%2Fsigs.5138968/MediaObjects/40793_2015_9031076_Fig1.jpg)
Phylogenetic tree highlighting the position of M. limicola relative to the type strains of the other species within the family Methanomicrobiaceae. The tree was inferred from 1,271 aligned characters of the 16S rRNA gene sequence under the maximum likelihood (ML) criterion and rooted as previously described [7]. The branches are scaled in terms of the expected number of substitutions per site. Numbers adjacent to the branches are support values from 250 ML bootstrap replicates [8] (left) and from 1,000 maximum-parsimony bootstrap replicates [9] (right) if larger than 60%. Lineages with type-strain genome sequencing projects registered in GOLD [10] are labeled with one asterisk, those also listed as ‘Complete and Published’ with two asterisks [11–14] (for Methanoregula boonei and Methanosphaerula palustris see CP000780 and CP001338, respectively).