Fig. 3
From: Complete genome sequence of the phenanthrene-degrading soil bacterium Delftia acidovorans Cs1-4
Antigenic relation of NpdA from Delftia acidovorans Cs1-4 with other members of Comamonadaceae family. (Left panel) SDS PAGE . (right panel) Immunoblot with anti-NpdA antibody. Lane 1, Molecular weight marker; lane 2, Delftia acidovorans Cs1-4 (phenanthrene-grown); lane 3, Delftia acidovorans SPH1; lane 4, Deftia acidovorans ATCC15688; lane 5, Delftia acidovorans Cs1-4 (nutrient broth-grown); lane 6, Acidovorax delafieldii 2AN; lane 7, Verminephrobacter eisenae EF01-2; Lane 8, M3. Each lane was loaded with 50 μg of protein, and the gel stained with Coomasie blue. Preparation of anti-NpdA IgY and immunoblotting procedures are described in Shetty et al. [2]