Fig. 4From: Comparing polysaccharide decomposition between the type strains Gramella echinicola KMM 6050T (DSM 19838T) and Gramella portivictoriae UST040801-001T (DSM 23547T), and emended description of Gramella echinicola Nedashkovskaya et al. 2005 emend. Shahina et al. 2014 and Gramella portivictoriae Lau et al. 2005Polysaccharide hydrolysis by Gramella type strains G. echinicola DSM 19838T, G. portivictoriae DSM 23547T. Both strains were incubated in medium 514 (6 g/L carbon source mix) and HaHa (12 mg/L carbon source mix) for up to 14 days. G. echinicola DSM 19838T was incubated at 25 °C and G. portivictoriae DSM 23547T at 28 °C. Each 200 μL well of a microtiter plate was filled with a small portion of one of the AZO-CL-polysaccharides, −casein (Megazym, Bray, Ireland), charcoal-pectin, −gelatin in 100 μL medium. Each well was inoculated with 100 μL of a starved culture of the strains. The control wells were inoculated with 100 μL medium. The blue colour indicates the release of AZO- monomers and thus hydrolysis of the polysaccharide/peptide. A red-brown colour indicates growth of the strain (mixture of blue and yellow-orange). Black grains in the surrounding of the charcoal-pectin and -gelatine indicate hydrolysisBack to article page