Fig. 1

Bacteriophage characterization. a. Lysis halo of fSE1C on S. Enteritidis lawn; b. TEM of fSE1C; c. Lysis halo of fSE4S on S. Enteritidis lawn; d. TEM of fSE4S; e. Restriction pattern of bacteriophage genomic DNA; f. Evolutionary relationships of fSE1C and fSE4S bacteriophages; light red: Jerseyvirus; violet: Sp3unalikevirus; blue: K1glikevirus; green: current isolated phages members of the Jerseyvirus genus; The evolutionary history was inferred using the Neighbor-Joining method [23]. The optimal tree with the sum of branch length = 2.55835582 is shown. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the p-distance method [25] and are in the units of the number of base differences per site. The analysis involved 25 nucleotide sequences. All ambiguous positions were removed for each sequence pair. There were a total of 104441 positions in the final dataset. Evolutionary analyses were conducted in MEGA6 [26]. g. fSE1C bacteriophage genome map; the unique gene to fSE1C is indicated in red and the putative cas4 gene in blue; h. fSE4S bacteriophage genome map; the putative cas4 gene is indicated in blue. The internal circle show the G + C % in red and the A + T % in black. DNAPlotter was utilized for genome map visualization [33]