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Table 5 Illumina assembly statistics for each genome. Contig number and assembly length are from extracted bins. Illumina coverage calculated by mapping with BWA-MEM. Bin coverage parameters used to bin Illumina assemblies with mmgenome

From: Towards long-read metagenomics: complete assembly of three novel genomes from bacteria dependent on a diazotrophic cyanobacterium in a freshwater lake co-culture

 

Illumina coverage

# Illumina contigs

Bin coverage parameters

Bin assembly length (bp)

Bin assembly (% of genome)

Bin estimated completeness

Bin estimated contamination

Hyphomonadaceae UKL13-1

23x

122

Illumina: 15-40x PacBio: >49x

3,716,244

106.13%

98.48%

2.19%

Betaproteobacterium UKL13-2

63x

162

Illumina: 37-87x PacBio: 71-211x

3,131,899

92.47%

96.15%

1.42%

Bacteroidetes bacterium UKL13-3

58x

96

Illumina: 44-103x PacBio: >228x

3,009,740

92.99%

97.81%

0.55%

  1. Assembly as % of genome is comparison of contig bin length with actual genome length. Completeness and contamination estimated with CheckM