Fig. 4
From: Automation of PacBio SMRTbell NGS library preparation for bacterial genome sequencing
Appearance of sheared DNA from Agilent 2100 Bioanalyzer analysis. Representative electropherogram (a) and virtual gel (b) are used for visual inspection (generated with the Agilent 2100 Bioanalyzer system with the DNA 12000 Kit) of sheared bacterial genomic DNA with average shearing size for Campylobacter (green, 10 kb), Listeria (blue, 13.5 kb), Vibrio (aqua,11.6 kb), and Salmonella (red, 17 kb). Peaks near 35 are the lower marker internal standard for the DNA 12000 kit. A typical electropherogram using the Agilent Bioanalyzer 2100 DNA 12000 kit shows the lower marker at 35 s and the upper marker at 90 s. The sheared DNA and the red upper marker, seen in the gel image, co-elute together