Fig. 2

Transmission Electron Microscopy negative stain of Paraburkholderia caballeronis TNe-841^T. The strain was grown on LB medium and a loop-full of cells was gently suspended in 1 mL distilled water. A drop of the suspension was placed on a formvar-coated copper grid and air-dried for 20 min to allow the cells to adhere. The grid was then covered for 20 s with a solution of 0.5% uranyl acetate, the excess liquid was removed with a filter paper, and then air-dried. A JEOL JEM-1010 transmission electron microscope, operated at 60 kV, was used to observe and photograph negatively stained preparations. F, stands for flagella