Fig. 3

Symbiotype groups harbor unique communities of bacteria and fungi. Constrained analysis of principal coordinates (CAP) on Bray-Curtis dissimilarities of all samples analyzed and the factors presence/absence of rhizobia, presence/absence of AMF, and plant species as explanatory terms in bacterial (A) and fungal (B) datasets without the primary symbionts. The percentage of total variance explained by each CAP axis is given in parentheses. Ellipses represent the 95% confidence interval of the group centroids. Heatmap values reflect the z-scores of the DESeq2 normalized OTU abundance values for the bacterial (C) and fungal (D) symbiotype specific OTU communities for symbiotypes N, A, and AR. The total number of enriched OTUs (log₂FC > 1, adjusted p (FDR) < 0.05) for each symbiotype is indicated below each symbiotype name. Taxonomy assignments and normalized abundance values of all ssOTUs in the individual plant species and symbiotype groups are presented in Additional file 8