Fig. 5
From: Successional action of Bacteroidota and Firmicutes in decomposing straw polymers in a paddy soil
Properties of CGCs encoded in the Bacteroidota and Firmicutes MAGs. A, B The maximum likelihood phylogenetic tree reconstructed based on 120 concatenated marker genes (left panel), targeted substrate prediction of CGCs (middle panel) and the total numbers of CAZymes and GH proteins (right panel) encoded in the individual MAGs of Bacteroidota (A) and Firmicutes (B), respectively. Full description of CGCs and detected proteins are given in Table S1. Targeted substrates are indicated by closed circles and colored by substrate identity. Different lineage clades in phylogenetic trees are colored by taxonomic order (Bacteroidota) or class (Firmicutes). C The average numbers of predicted CGCs with and without targeted substrates in Bacteroidota and Firmicutes. Notably, very limited proportions of predicted CGCs had the matched substrates in dbCAN-PUL database. D The proportion of GHs with signal peptides in Bacteroidota and Firmicutes. E Spearman rank correlations (one-tailed) between the number of predicted CGCs and the number of glycoside hydrolases (blue, Bacteroidota; red, Firmicutes). The solid colored line indicates the fitted one-tailed Spearman correlation (R2), and the grey bands indicate the corresponding 95% confidence intervals for the curve. The regression equations and correlation coefficients are shown if statistically significant (p < 0.05). Abbreviations: CGC, CAZymes gene cluster; MAG, metagenomic assembled genome; GH, glycoside hydrolase