Table 1 Primer sets used for ddPCR
Primer | Primer target | Primer sequence (5′–3′) | Amplicon (bp) | References |
|---|---|---|---|---|
β-actin_Alor_F | Acropora loripes β-actin | TTGTTCTGGCTGGGGGAAAC | 101 | This study |
β-actin_Alor_R | GTTTTGATGGTGCTTGGGGC | |||
967F | Bacterial 16S rRNA gene | CAACGCGAAGAACCTTACC | 79 | [63] |
1046R | CGACAGCCATGCANCACCT | |||
En 667F | Endozoicomonas spp. 16S rRNA gene | CTAGAGTGCGGAAGAGGAGT | 104 | [64] |
mEn771R | TCAGTGTCARRCCAGAGTGT |