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Table 2 α- and β-diversity analysis results by coral species

From: DNA from non-viable bacteria biases diversity estimates in the corals Acropora loripes and Pocillopora acuta

Species

Rarefaction

α-Diversity

β-diversity

Observed ASVs (mean ± SE)

Inverse Simpson

Shannon

PERMANOVA (weighted UniFrac matrix)

A. loripes

737 (removed 106 ASVs)

NA

24 ± 2 (n = 46)

PMA

(F(1,35) = 12.52, p = 0.0012)

PMA

(F(1,35) = 15.75, p = 0.0003)

PMA

(F(1,45) = 24.15, p = 0.0001)

Figure 1A

Figure 1B

Figure 1C

Figure 2A, B

A. millepora

2184 (removed 6 samples and 309 ASVs)

NA

50 ± 10 (n = 43)

NA

Genotype

(F(3,34) = 6.44, p = 0.0014)

NA

Additional file 2: Fig. S4A

A. kenti

9895 (Removed 3 samples and 151 ASVs)

Genotype

(F(3,29) = 8.24, p = 0.0004)

A-568 ± 113 (n = 9)

B-213 ± 39 (n = 10)

C-211 ± 55 (n = 8)

D-135 ± 23 (n = 10)

Genotype

(F(3,29) = 15.43, p < 0.0001)

Genotype

(F(3,29) = 14.27, p < 0.0001)

Genotype

(F(3,39) = 11.52, p = 0.001)

Additional file 2: Fig. S3A

Additional file 2: Fig. S3B

Additional file 2: Fig. S3C

Additional file 2: Fig. S4B

P. acuta

883 (removed 2 samples and 66 ASVs)

NA

20 ± 2 (n = 48)

NA

NA

PMA

(F(1,49) = 1.88, p = 0.0106)

Figure 2C-D

P. daedalea

10,489 (removed 5 samples and 243 ASVs)

Genotype

(F(4,31) = 3.17, p = 0.0271)

A-293 ± 17 (n = 10)

B-234 ± 50 (n = 10)

C-76 ± 18 (n = 8)

D-178 ± 76 (n = 9)

E-75 ± 46 (n = 4)

Genotype

(F(4,31) = 8.46, p = 0.0001)

Genotype

(F(4,31) = 3.40, p = 0.0203)

Genotype

(F(4,45) = 52.88, p = 0.001)

Additional file 2: Fig. S3D

Additional file 2: Fig. S3E

Additional file 2: Fig. S3F

Additional file 2: Fig. S4C

P. lutea

4390 (removed 9 samples and 302 ASVs)

NA

180 ± 22 (n = 41)

NA

NA

Genotype

(F(4,49) = 3.30, p = 0.001)

Additional file 2: Fig. S4D

  1. Rarefaction levels were chosen based on rarefaction curves for Observed ASVs (Additional file 2: Fig. S2)
  2. A significant main factor effect is denoted by “PMA” or “Genotype;” there were no interactions between main effects. Where there were significant differences, statistics are provided as well as the figure where a given data type is visualized