Fig. 3

Taxa occurring in different proportions of surface water samples taken on shared sample dates between BML and BCR for gene sequencing data (0.3–0.6 m depth) and cell count data (0.6–0.8 m depth) over all years. Taxa for the gene sequencing data were ASVs, while those for the cell counts were species. For each set of bars, the lighter shades indicate ephemeral taxa (present in few samples), while the darker shades indicate increasingly more persistent taxa (present in most samples). BML showed more ephemeral and fewer persistent taxa than BCR. Data were normalized using scaling with ranked subsampling (SRS) [41] to 4000, 1000, 100, and 10,000 for the 23S, 18S, and 16S rRNA gene datasets and cell count data, respectively. Each bar represents the mean of three replicates ± 1 SEM. No replicates were available for BCR cell count data