Fig. 6

Relative abundances of the four major algal genera in BML surface waters: A Choricystis, B Cryptomonas, C Synechococcus, and D Euglena, based on 23S, 16S and/or 18S rRNA gene amplicon sequencing. Non-cyanobacteria were filtered from the 16S rRNA gene dataset and known non-photosynthetic eukaryotes were filtered from the 18S rRNA gene dataset. Also included for B) and D) are microscopic cell count data. Note that the different analyses are not fully comparable due to their respective limitations and biases (see Discussion: Gene Sequencing and Microscopy Complementarity). Data points are means of three platforms ± 1 SEM. Where error bars are not seen they are contained within the symbol. The teal bar indicates alum addition. Dashed lines indicate running averages calculated with smoothing in SigmaPlot, in which a running average is based on 10 samples. In panel B, the single extreme outlier in the microscopic count data was not used in the running average