Fig. 1
From: Exploring virus-host-environment interactions in a chemotrophic-based underground estuary
Viral abundance, diversity, and novelty in Bundera Sinkhole. (a) Topology of the sinkhole and sampling points for shotgun metagenomic sequencing. Figure panel adapted from Elbourne, et al. [20]. (b) Total relative abundance (% of metagenome) of all viruses in each sample, calculated by the transcripts per million (TPM) sum of all viral contigs divided by 1 million (i.e., the TPM sum of all contigs). (c) Relative abundance (TPM) of vOTUs within each sample. Colour scale is displayed as log10(TPM + 1) to account for TPM values of zero. (d) Venn diagram showing the number of unique and shared vOTUs (italicised numbers) among the 2 m (blue), 8 m (yellow), and 17-28 m (red) clusters. (e) Relationship between water column depth and vOTU alpha diversity (Shannon index). (f) Estimation of viral novelty based on vOTU nucleotide sequence homology with the IMG/VR v3 database [49]. vOTUs were classified based on > 90% identity (known virus), 50–90% identity (similar virus), and < 50% identity (novel virus) over at least 70% of either query or database sequence