Fig. 1
From: Microbiome changes through the ontogeny of the marine sponge Crambe crambe
Pictures of all the Crambe crambe ontogenetic stages analysed (A–D) and ultrastructural images of their tissue and associated microbes E–G. A Adult individual of Crambe crambe in the field. B Brooded larvae (arrows) within the maternal tissue and free-swimming larva (inset). Scale bar: 1 mm for embryos and 0.5 mm for the free-swimming larva (inset). C. Settled juvenile without an osculum (JNO). Scale bar: 1 mm. D Settled juvenile with an osculum (JO). Note the osculum in the upper part of the juvenile sponge (arrow). Scale bar: 1 mm. E Mesohyl in the adult sponge showing a variety of symbionts (arrows), with a bacterium morphotype more abundant than the others (s). F Larval flagellated epithelium showing very few symbionts (arrows) in the intercellular medium, except for a common bacterial morphotype (s), which also occurrs in the adult (E). G Mesohyl of a juvenile with osculum showing few symbionts (arrows), with a common bacterium morphotype (s) similar to that occurring in adults and larvae (E and F)