Figure 1.
From: VIROME: a standard operating procedure for analysis of viral metagenome sequences
Overview flow-chart of VIROME bioinformatic pipeline. A) Initial screening steps to remove poor quality sequences, false duplicate sequences created during 454 em-PCR library preparation, and rRNA-containing sequences. Contaminating sequence screens includes searches against the UniVec database for vector, linker, and adapter sequences. B) Analysis steps including the identification of tRNA-containing sequences and BLASTP of metagenome peptides against the UniRef 100 and MGOL sequence databases. Significant BLASTP hits have an expectation score of E <0.001. C) Viral metagenome peptide sequences with a significant hit a UniRef 100 sequences are characterized by the taxonomic origin of the homolog and functional information contained within UniRef or the annotated databases. Those metagenome peptides with hits to the MGOL database are characterized according to the environmental origin of their MGOL homologs (Figure 3). Sequences within blue objects are accessible through VIROM web-application interface for viewing or download. Parameters for sequence analyses (rectangles) are given in Table 1.