Figure 4.
From: VIROME: a standard operating procedure for analysis of viral metagenome sequences
Flow-chart of VIROME environmental annotation. For each predicted viral metagenome ORF, E-scores (E<0.01) of top-hits against each unique library in the MGOL database are summed. Ratios of E-score distribution for each unique MGOL library are calculated. These ratios can be used to examine the prevalence of sequence homologs according to the environmental features of MGOL libraries (e.g., ecosystem, biome, physico-chemical parameters).