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Table 1. Algorithms, parameters, and databases used in the VIROM bioinformatics pipeline

From: VIROME: a standard operating procedure for analysis of viral metagenome sequences

Process

Tool

Parameters

Subject database

Screening of rRNAs

BLASTALL

-p blastn −e 1e-3 −f T − b 1 −v 1 −M BLOSUM62

 

Identification of tRNAs

tRNA scan SE

-b G

 

ORF calling

MetaGene Annotator

-m

 

Known protein identification

BLASTALL

-p blastp −e 1e-1 −F T − b 50 −v 50 −M BLOSUM 62

UNIREF 100

Environmental Protein Identification

BLASTALL

-p blastp −e 1e-1 −F T −b 50 −v 50 −M BLOSUM62

MGOL