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Table 2. BLASTP evidence classes.

From: The JCVI standard operating procedure for annotating prokaryotic metagenomic shotgun sequencing data

BLAST Hit Class

Annotative meaning

High Confidence

35% identity or greater, across 85% or more of the length

Putative

less than 35% identity, but across 80% or more of the length

Conserved Domain

35% identity or greater, but across less than 80% of the length

Low Confidence

less than 35% identity across less than 80% of the length

  1. These have been established through extensive manual curation and validation efforts, and are ordered by trustworthiness.