Open Access

Complete genome sequence of Anaerococcus prevotii type strain (PC1T)

  • Kurt LaButti1,
  • Rüdiger Pukall2,
  • Katja Steenblock2,
  • Tijana Glavina Del Rio1,
  • Hope Tice1,
  • Alex Copeland1,
  • Jan-Fang Cheng1,
  • Susan Lucas1,
  • Feng Chen1,
  • Matt Nolan1,
  • David Bruce1, 3,
  • Lynne Goodwin1, 3,
  • Sam Pitluck1,
  • Natalia Ivanova1,
  • Konstantinos Mavromatis1,
  • Galina Ovchinnikova1,
  • Amrita Pati1,
  • Amy Chen4,
  • Krishna Palaniappan4,
  • Miriam Land1, 5,
  • Loren Hauser1, 5,
  • Yun-Juan Chang1, 5,
  • Cynthia D. Jeffries1, 5,
  • Patrick Chain1, 6,
  • Elizabeth Saunders1, 3,
  • Thomas Brettin1, 3,
  • John C. Detter1, 3,
  • Cliff Han1, 3,
  • Markus Göker2,
  • Jim Bristow1,
  • Jonathan A. Eisen1, 7,
  • Victor Markowitz4,
  • Philip Hugenholtz1,
  • Nikos C. Kyrpides1,
  • Hans-Peter Klenk2 and
  • Alla Lapidus1
Standards in Genomic Sciences20091:1020159

DOI: 10.4056/sigs.24194

Published: 29 September 2009

Abstract

Anaerococcus prevotii (Foubert and Douglas 1948) Ezaki et al. 2001 is the type species of the genus, and is of phylogenetic interest because of its arguable assignment to the provisionally arranged family ‘Peptostreptococcaceae’. A. prevotii is an obligate anaerobic coccus, usually arranged in clumps or tetrads. The strain, whose genome is described here, was originally isolated from human plasma; other strains of the species were also isolated from clinical specimen. Here we describe the features of this organism, together with the complete genome sequence and annotation. This is the first completed genome sequence of a member of the genus. Next to Finegoldia magna, A. prevotii is only the second species from the family ‘Peptostreptococcaceae’ for which a complete genome sequence is described. The 1,998,633 bp long genome (chromosome and one plasmid) with its 1852 protein-coding and 61 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.

Keywords

Firmicutes Clostridiales Peptostreptococcaceae Gram-positive coccoid human oral microflora skin non-motile non-sporulating anaerobic

Introduction

Anaerococcus prevotii strain PC1T (= DSM 20548 = ATCC 9321 = JCM 6508) is the type strain of the species and the type species of the genus [1]. Six strains of the species were characterized by Foubert and Douglas in 1948, originally designated as ‘Micrococcus prevotii’, but subsequently placed in the genus Peptococcus [2]. Based on a comparative study published by Ezaki et al. [3], the type strain of P. prevotii was then transferred to the genus ‘Peptostreptococcus’ and later on assigned to the novel genus Anaerococcus as A. prevotii [1]. The organism is a Gram-positive, anaerobic, indole-negative coccus. The major metabolic end product from metabolism of peptone-yeast-glucose (PYG) is butyric acid. A. prevotii was provisionally assigned to the arranged family ‘Peptostreptococcaceae’ within the order Clostridiales, also designated as Family XI Incertae sedis [4]. Here we present a summary classification and a set of features for A. prevotii strain PC1T together with the description of the complete genomic sequencing and annotation.

Classification and features

Within the last few years, several changes occurred in the classification of the anaerobic Gram-positive cocci. There are currently five genera of anaerobic Gram-positive cocci which may be isolated from humans (Peptostreptococcus, Peptoniphilus, Parvimonas, Finegoldia, and Anaerococcus). Members of the species A. prevotii are frequently recovered from human clinical specimens such as vaginal discharges and ovarian, peritoneal, sacral or lung abscesses. In particular, A. prevotii was also described as a common isolate of the normal flora of skin, the oral cavity and the gut [3]. Historically the Gram-positive anaerobic cocci were identified mainly by using phenotypic traits, but as shown by Song et al., this often led to the misidentification of A. vaginalis strains, which were mistakenly assigned to A. prevotii or A. tetradius [5]. Currently Genbank does not contain any16S rRNA sequences from cultivated strains that can be clearly linked to the species A. prevotii with over 95% gene sequence similarity. Recently, the temporal diversity of the human skin microbiome was analyzed using 16S rRNA gene phylotyping. It is noteworthy that several clones originated from different skin sites (gluteal crease, occiput, umbilicus, popliteal fossa, volar forearm). These isolates were taken from two patients and showed close relationships to A. prevotii [6]. No closely related isolates or uncultivated clones with more than 84% 16S rRNA gene sequence identity are recorded from global ocean screenings and environmental samples (except for human skin).

Figure 1 shows the phylogenetic neighborhood of A. prevotii strain PC1T in a 16S rRNA based tree. The four 16S rRNA gene copies in the genome of strain PC1T differ by up to 15 nucleotides from each other, and by up to 9 nucleotides from the previously published 16S rRNA sequence generated from strain CCUG 41932 (AF542232). The difference between the genome data and the reported 16S rRNA gene sequence is most likely due to sequencing errors in the previously reported sequence data.
Figure 1.

Phylogenetic tree highlighting the position of A. prevotii PC1T relative to all type strains of the genus Anaerococcus and the type strains of all other genera within the family ‘Peptostreptococcaceae’ inferred from 1,302 aligned characters [7,8] of the 16S rRNA sequence under the maximum likelihood criterion [9]. Rooting was done with the type species of the genus Clostridium. The branches are scaled in terms of the expected number of substitutions per site. Numbers above branches are support values from 1,000 bootstrap replicates if larger than 60%. Lineages with type strain genome sequencing projects registered in GOLD [10] are shown in blue, published genomes in bold.

A. prevotii PC1T cells are Gram-positive and non-motile(Table 1). Cells grown in PYG broth are 0.6–0.9 µm in diameter and occur in pairs, tetrads or irregular clumps or short chains (Figure 2). Colonies range from 0.5 to 2 mm in diameter on Columbia blood agar. Optimum temperature for growth is 37°C. Strain PC1T metabolizes peptones and amino acids and the major metabolic end product from PYG medium is butyric acid. Most species of the genus Anaerococcus ferment carbohydrates weakly. A. prevotii is proteolytic. α-Glucosidase, α-galactosidase, ß-glucuronidase and pyroglutamyl arylamidase activities are detectable [19,20]. Production of urease may vary among strains of the species. Most strains produce ammonia from threonine and serine [3] by deamination of the amino acids to pyruvate. A. prevotii is resistant to sodium polyanethol sulfonate [21], but susceptible to the penicillins [19].
Table 1.

Classification and general features of A. prevotii PC1T in accordance with the MIGS recommendations [11]

MIGS ID

Property

Term

Evidence code

 

Current classification

Domain Bacteria

TAS [12]

 

Phylum Firmicutes

TAS [13]

 

Class Clostridia

TAS [4]

 

Order Clostridiales

TAS [14]

 

Family ‘Peptostreptococcaceae

TAS [4]

 

Genus Anaerococcus

TAS [1]

 

Species Anaerococcus prevotii

TAS [1]

 

Type strain PC1

TAS [1,3]

 

Gram stain

positive

TAS [15]

 

Cell shape

coccoid

TAS [15]

 

Motility

nonmotile

TAS [15]

 

Sporulation

nonsporulating

TAS [15]

 

Temperature range

mesophile

TAS [15]

 

Optimum temperature

37°C

TAS [15]

 

Salinity

growth in PYG +6% NaCl

TAS [16]

MIGS-22

Oxygen requirement

anaerobic

TAS [15]

 

Carbon source

unknown

 
 

Energy source

peptones

TAS [1,3]

MIGS-6

Habitat

human mouth, skin and vaginal microflora

TAS [3,10]

MIGS-15

Biotic relationship

free living

NAS

MIGS-14

Pathogenicity

opportunistic infections

TAS [10]

 

Biosafety level

2

TAS [17]

 

Isolation

human plasma

TAS [3]

MIGS-4

Geographic location

not reported

 

MIGS-5

Sample collection time

not reported

 

MIGS-4.1

   

MIGS-4.2

Latitude - Longitude

not reported

 

MIGS-4.3

Depth

not reported

 

MIGS-4.4

Altitude

not reported

 

Evidence codes - IDA: Inferred from Direct Assay (first time in publication); TAS: Traceable Author Statement (i.e., a direct report exists in the literature); NAS: Non-traceable Author Statement (i.e., not directly observed for the living, isolated sample, but based on a generally accepted property for the species, or anecdotal evidence). These evidence codes are available from the Gene Ontology project [18]. If the evidence code is IDA, then the property was directly observed for a live isolate by one of the authors or an expert mentioned in the acknowledgements.

Chemotaxonomy

Cell wall amino acid analysis of strain PC1T yielded peptidoglycan type A4α′, composed of L-Lys-D-Glu [22], type A12.2 according to the DSMZ catalogue of strains. Cell wall sugars are glucose, glucosamine and galactose [22]. Major cellular fatty acid composition of the type strain was analyzed by Lambert and Armfield in 1979 [23] and by Ezaki et al. in 1983 [3], but the results of these studies are contradictory. No other chemotaxonomic data are available at present.

Genome sequencing and annotation

Genome project history

This organism was selected for sequencing on the basis of its phylogenetic position, and is part of the Genomic Encyclopedia of Bacteria and Archaea project. The genome project is deposited in the Genomes OnLine Database [10] and the complete genome sequence has been deposited in GenBank. Sequence, finishing and annotation were performed by the DOE Joint Genome Institute (JGI). A summary of the project information is shown in Table 2.
Table 2.

Genome sequencing project information

MIGS ID

Property

Term

MIGS-31

Finishing quality

Finished

MIGS-28

Libraries used

Three genomic libraries: two Sanger libraries - 8 kb pMCL200 and fosmid pcc1Fos - and one 454 pyrosequence standard library

MIGS-29

Sequencing platforms

ABI3730, 454 GS FLX, Illumina GA

MIGS-31.2

Sequencing coverage

6.8 Sanger; 42.3 pyrosequence

MIGS-30

Assemblers

Newbler version 1.1.02.15, Arachne

MIGS-32

Gene calling method

Prodigal, GenePRIMP

 

Genbank IDs

CP001708 (chromosome)

  

CP001709 (plasmid pAPRE01)

 

Genbank Dates of Release

4/28/2009 (chromosome)

  

4/28/2009 (plasmid pAPRE01)

 

GOLD ID

Gc01089

 

NCBI project ID

29533

 

Database: IMG-GEBA

2501533213

MIGS-13

Source identifier

DSM 20548

 

Project relevance

Tree of Life, GEBA

Growth conditions and DNA isolation

A. prevotii strain PC1T, DSM 20548, was grown anaerobically in DSMZ medium 104 [24] at 37°C. DNA was isolated from 1–1.5 g of cell paste using Qiagen Genomic 500 DNA Kit (Qiagen, Hilden, Germany) following the instructions given by the manufacturer, but with a modified protocol for cell lysis, LALMP, according to Wu et al. [25].

Genome sequencing and assembly

The genome was sequenced using a combination of Sanger, 454 and Illumina sequencing platforms. All general aspects of library construction and sequencing can be found at the JGI web site. Reads produced by 454 Pyrosequencing were assembled using the Newbler assembler version 1.1.02.15 (Roche). Large Newbler contigs were broken into 2,196 overlapping fragments of 1,000 bp and entered into the assembly as pseudo-reads. The sequences were assigned quality scores based on Newbler consensus q-scores with modifications to account for overlap redundancy and to adjust inflated q-scores. A hybrid 454/Sanger assembly was made using the Arachne assembler. Possible mis-assemblies were corrected and gaps between contigs were closed by custom primer walks from sub-clones or PCR products. A total of 66 Sanger finishing reads were produced. Illumina reads were used to improve the final consensus quality using an in-house developed tool (the Polisher). The final assembly consisted of 18,576 Sanger and 464,157 Roche/454 reads. The error rate of the completed genome sequence is less than 1 in 100,000. Together all sequence types provided 49.1 coverage of the genome.

Genome annotation

Genes were identified using Prodigal [26] as part of the Oak Ridge National Laboratory genome annotation pipeline, followed by a round of manual curation using the JGI GenePRIMP pipeline [27]. The predicted CDSs were translated and used to search the National Center for Biotechnology Information (NCBI) nonredundant database, UniProt, TIGRFam, Pfam, PRIAM, KEGG, COG, and InterPro databases. Additional gene prediction analysis and functional annotation was performed within the Integrated Microbial Genomes (IMG-ER) platform [28].

Genome properties

The genome is 1,998,633 bp long (chromosome and one circular plasmid) with a 35.6% GC content (Table 3). Of the 1,913 genes predicted, 1,852 were protein coding genes, and 61 were RNAs. A total of 46 pseudogenes were also identified, with 73.1% of the genes being assigned a putative function. The remaining genes were annotated as hypothetical proteins. The distribution of genes into COGs functional categories is presented in Figure 3 and Table 4.
Figure 2.

Scanning electron micrograph of A. prevotii PC1T (M. Rohde, HZI Braunschweig)

Figure 3.

Graphical circular map of the genome. From outside to the center: Genes on forward strand (color by COG categories), Genes on reverse strand (color by COG categories), RNA genes (tRNAs green, sRNAs red, other RNAs black), GC content, GC skew.

Table 3.

Genome Statistics

Attribute

Value

% of Total

Genome size (bp)

1,998,633

100.00%

DNA Coding region (bp)

1,815,671

90.85%

DNA G+C content (bp)

712,291

35.64%

Number of replicons

2

 

Extrachromosomal elements

1

 

Total genes

1913

100.00%

RNA genes

61

3.19%

rRNA operons

4

 

Protein-coding genes

1852

96.81%

Pseudo genes

46

2.405%

Genes with function prediction

1399

73.13%

Genes in paralog clusters

231

12.08%

Genes assigned to COGs

1421

74.28%

Genes assigned Pfam domains

1428

74.65%

Genes with signal peptides

337

17.62%

Genes with transmembrane helices

467

24.41%

CRISPR repeats

0

 
Table 4.

Number of genes associated with the 21 general COG functional categories

Code

Value

%

Description

J

133

7.2

Translation, ribosomal structure and biogenesis

A

0

0.0

RNA processing and modification

K

118

6.4

Transcription

L

105

5.7

Replication, recombination and repair

B

1

0.1

Chromatin structure and dynamics

D

20

1.1

Cell cycle control, mitosis and meiosis

Y

0

0.0

Nuclear structure

V

55

3.0

Defense mechanisms

T

43

2.3

Signal transduction mechanisms

M

69

3.7

Cell wall/membrane biogenesis

N

5

0.3

Cell motility

Z

0

0.0

Cytoskeleton

W

0

0.0

Extracellular structures

U

19

1.0

Intracellular trafficking and secretion

O

61

3.3

Posttranslational modification, protein turnover, chaperones

C

84

4.5

Energy production and conversion

G

144

7.8

Carbohydrate transport and metabolism

E

107

5.8

Amino acid transport and metabolism

F

61

3.3

Nucleotide transport and metabolism

H

56

3.0

Coenzyme transport and metabolism

I

37

2.0

Lipid transport and metabolism

P

102

5.5

Inorganic ion transport and metabolism

Q

9

0.5

Secondary metabolites biosynthesis, transport and catabolism

R

84

4.5

General function prediction only

S

118

6.4

Function unknown

-

431

23.3

Not in COGs

Declarations

Acknowledgements

We would like to gratefully acknowledge the help of Susanne Schneider (DSMZ) for DNA extraction and quality analysis. This work was performed under the auspices of the US Department of Energy Office of Science, Biological and Environmental Research Program, and by the University of California, Lawrence Berkeley National Laboratory under contract No. DE-AC02-05CH11231, Lawrence Livermore National Laboratory under Contract No. DE-AC52-07NA27344, and Los Alamos National Laboratory under contract No. DE-AC02-06NA25396, as well as German Research Foundation (DFG) INST 599/1-1.

Authors’ Affiliations

(1)
DOE Joint Genome Institute
(2)
DSMZ - German Collection of Microorganisms and Cell Cultures GmbH
(3)
Bioscience Division, Los Alamos National Laboratory
(4)
Biological Data Management and Technology Center, Lawrence Berkeley National Laboratory
(5)
Oak Ridge National Laboratory
(6)
Lawrence Livermore National Laboratory
(7)
University of California Davis Genome Center

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Copyright

© The Author(s) 2009