Open Access

Non contiguous-finished genome sequence and description of Enorma timonensis sp. nov.

  • Dhamodaran Ramasamy1,
  • Gregory Dubourg1,
  • Catherine Robert1,
  • Aurelia Caputo1,
  • Laurent Papazian1, 2,
  • Didier Raoult1, 3 and
  • Pierre-Edouard Fournier1Email author
Standards in Genomic Sciences20149:9030970

DOI: 10.4056/sigs.4878632

Published: 15 June 2014

Abstract

Enorma timonensis strain GD5T sp. nov., is the type strain of E. timonensis sp. nov., a new member of the genus Enorma within the family Coriobacteriaceae. This strain, whose genome is described here, was isolated from the fecal flora of a 53-year-old woman hospitalized for 3 months in an intensive care unit. E. timonensis is an obligate anaerobic rod. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 2,365,123 bp long genome (1 chromosome but no plasmid) contains 2,060 protein-coding and 52 RNA genes, including 4 rRNA genes.

Keywords

Enorma timonensis genome culturomics taxono-genomics

Introduction

Enorma timonensis strain GD5T (= CSUR P900 = DSM 26111) is the type strain of E. timonensis sp. nov. This bacterium was isolated from the stool of a 53-year-old French woman hospitalized for 3 months into an intensive care unit for a Guillain-Barre syndrome, as part of a culturomics study aiming at cultivating individually all species within human feces [13]. It is a Gram-positive, anaerobic, non-endospore forming, indole-negative, rod-shaped bacillus.

The human gut microbiota consists of billions of microorganisms that outnumber the human cells [4]. Advances in DNA sequence-based technologies and the development of 16S ribosomal RNA sequence-based metagenomic methods have been used to explore the complex gut microbial population, which has a crucial role in human health and disease development [5,6]. The currently used strategy for determining the taxonomic status of a bacterial isolate includes comparing it to its phylogenetically closest neighbors in terms of 16S rRNA gene similarity, G + C content and DNA-DNA hybridization (DDH) [7,8]. However, although considered “gold standards” in bacterial taxonomy, these criteria do not apply to all genera [9,10]. The development of high-throughput sequencing methods [11] enabled the generation of complete genomic sequences for most bacterial species of medical interest (more than 6,000 bacterial genomes sequenced to date). We recently proposed to describe new bacterial species using a polyphasic approach based on their genome sequence, MALDI-TOF spectrum and main phenotypic characteristics [1234].

Here, we present a summary classification and a set of features for E. timonensis sp. nov. strain GD5T (= CSUR P900 = DSM 26111) as well as the description of the complete genome sequencing and annotation. These characteristics support the circumscription of the species E. timonensis.

The family Coriobacteriaceae (Stackebrandt et al. 1997) was created in 1997 [35] and presently consists of 13 validated genera [36]: Adlercreutzia (Maruo et al. 2008) [37], Asaccharobacter (Minamida et al. 2008) [38], Atopobium (Collins and Wallbanks 1993) [39], Collinsella (Kageyama et al. 1999) [40], Coriobacterium (Haas and König 1988) [41], Cryptobacterium (Nakazawa et al. 1999) [42], Denitrobacterium (Anderson et al. 2000) [43], Eggerthella (Wade et al. 1999) [44], Entherorhabdus (Clavel et al. 2009) [45], Gordonibacter (Würdemann et al. 2009) [46], Olsenella (Dewhirst et al. 2001) [47], Paraeggerthella (Würdemann et al. 2009) [46], Slackia (Wade et al. 1999) [44], and the recently described new genus Enorma (Mishra et al. 2013) [29]. These microorganisms are anaerobic, Gram-positive, rod-shaped bacteria [42]. Members of the family Coriobacteriaceae are isolated from the fecal microbiota of humans or animals, and may cause infections such as bacteremia, wound infections and periodontal/endodontic infections. Members of this family also interfere with the metabolism of triglycerides, glucose, and glycogen in humans and animals [3547].

Classification and features

A stool sample was collected from a 53-year-old woman living in Marseille, France and hospitalized for 3 months in an intensive care unit for Guillain-Barre syndrome. She received antibiotics at the time of stool sample collection. The patient gave an informed and signed consent, and the agreement of the local ethics committee of the Institut Federatif de Recherche 48 (Marseille, France) was obtained under agreement 09-022. The fecal specimen was preserved at −80°C after collection. Strain GD5T (Table 1) was isolated in 2012 by anaerobic cultivation at 37°C on 5% sheep blood-enriched Columbia agar (BioMerieux, Marcy l’Etoile, France), after 3 weeks of preincubation of the stool sample with clarified and sterile sheep rumen in an anaerobic blood culture bottle.
Table 1.

Classification and general features of Enorma timonensis strain GD5T according to the MIGS recommendations [48]

MIGS ID

Property

Term

Evidence codea

 

Current classification

Domain Bacteria

TAS [49]

 

Phylum Actinobacteria

TAS [50,51]

 

Class Actinobacteria

TAS [35]

 

Order Coriobacteriales

TAS [29,52,53]

 

Family Coriobacteriaceae

TAS [52,53]

 

Genus Enorma

TAS [53]

 

Species Enorma timonensis

IDA

 

Type strain GD5T

IDA

 

Gram stain

positive

IDA

 

Cell shape

rod

IDA

 

Motility

non motile

IDA

 

Sporulation

non sporulating

IDA

 

Temperature range

mesophile

IDA

 

Optimum temperature

37°C

IDA

MIGS-6.3

Salinity

unknown

IDA

MIGS-22

Oxygen requirement

anaerobic

IDA

 

Carbon source

unknown

NAS

 

Energy source

unknown

NAS

MIGS-6

Habitat

human gut

IDA

MIGS-15

Biotic relationship

free living

IDA

MIGS-14

Pathogenicity

Unknown

 
 

Biosafety level

2

 
 

Isolation

human feces

 

MIGS-4

Geographic location

France

IDA

MIGS-5

Sample collection time

January 2012

IDA

MIGS-4.1

Latitude

43.296482

IDA

MIGS-4.1

Longitude

5.36978

IDA

MIGS-4.3

Depth

Surface

IDA

MIGS-4.4

Altitude

0 m above sea level

IDA

aEvidence codes - IDA: Inferred from Direct Assay; TAS: Traceable Author Statement (i.e., a direct report exists in the literature); NAS: Non-traceable Author Statement (i.e., not directly observed for the living, isolated sample, but based on a generally accepted property for the species, or anecdotal evidence). These evidence codes are from the Gene Ontology project [54]. If the evidence is IDA, then the property was directly observed for a live isolate by one of the authors or an expert mentioned in the acknowledgements.

The 16S rDNA sequence (GenBank accession number JX424767) of E. timonensis strain GD5T exhibited the highest similarity (95.0%) with its phylogenetically closest published species, Enorma massiliensis (Figure 1). By comparison with the type species of genera from the family Coriobacteriaceae, E. timonensis exhibited a 16S rDNA sequence similarity ranging from 84 to 95%. This value was lower than the 98.7% 16S rDNA gene sequence threshold recommended by Stackebrandt and Ebers to delineate a new species without carrying out DNA-DNA hybridization [8].
Figure 1.

Phylogenetic tree highlighting the position of Enorma timonensis strain GD5T relative to other type strains within the Coriobacteriaceae family. GenBank accession numbers are indicated in parentheses. Sequences were aligned using CLUSTALW, and phylogenetic inferences obtained using the maximum-likelihood method within the MEGA software. Numbers at the nodes are percentages of 500 bootstrap replicates supporting that node. The tree is a majority consensus tree. Bifidobacterium bifidum was used as outgroup. The scale bar represents a 2% nucleotide sequence divergence.

Growth at different temperatures (25, 30, 37, 45°C) was tested. No growth was observed at 25°C or 30°C. Growth occurred at both 37 and 45°C, but optimal growth was observed at 37°C after 48 hours of incubation. Colonies were translucent grey and approximately 0.4 mm in diameter on 5% sheep blood-enriched Columbia agar (BioMerieux). Growth of the strain was tested in blood-enriched Columbia agar under anaerobic and microaerophilic conditions using GENbag anaer and GENbag microaer systems, respectively (BioMerieux), and under aerobic conditions, with or without 5% CO2. Growth was achieved only anaerobically. Gram staining showed Gram-positive and non-sporulated rods (Figure 2). A motility test was negative. Cells grown on agar have a mean diameter of 0.58 µm and a mean length of 1.32µm, and are mostly grouped in short chains or small clumps (Figure 3).
Figure 2.

Gram staining of E. timonensis strain GD5T.

Figure 3.

Transmission electron microscopy of E. timonensis strain GD5T using a Morgani 268D (Philips) at an operating voltage of 60kV. The scale bar represents 200µm.

Strain GD5T exhibited neither catalase nor oxidase activities (Table 2). Using an API ZYM strip (BioMerieux), positive reactions were observed for leucine arylamidase, valine arylamidase, cystin arylamidase, naphthol-AS-BI-phosphohydrolase, β-galactosidase, β-glucuronidase, α-glucosidase and β-glucosidase. Negative reactions were observed for acid phosphatase, nitrate reduction, urease alkaline phosphatase, esterase (C4), esterase lipase (C8), lipase (C14), trypsin, α-chemotrypsin, acid phosphatase, α-galactosidase, N-actetyl-β-glucosaminidase, α-mannosidase, α-fucosidase. Using an API Rapid ID 32A strip (BioMerieux), positive reactions were observed for proline arylamidase, phenylalanine arylamidase, histidin arylamidase, serine arylamidase. Negative reactions were observed for urease, arginine dihydrolase, tyrosin arylamidase, leucyl-glycyl arylamidase, alanine arylamidase, glycine arylamidase and arginine arylamidase. Using an API 50 CH strip (BioMerieux), negative reactions were recorded for fermentation of glycerol, erythritol, D-arabinose, L-arabinose, D-ribose, D-xylose, L-xylose, D-adonitol, methyl-βD-xylopranoside, D-galactose, D-glucose, D-fructose, D-mannose, L-sorbose, L-rhamnose, dulcitol, inositol, D-mannitol, D-sorbitol, methyl-α-D-xylopyranoside, methyl-α-D-glucopyranoside, N-acetylglucosamine, amygdalin, arbutin, esculin ferric citrate, salicin, D-cellobiose, D-maltose, D-lactose, D-mellibiose, D-saccharose, D-trehalose, inulin, D-melezitose, D-raffinose, amidon, glycogen, xylitol, gentiobiose, D-turanose, D-lyxose, D-tagatose, D-fucose, L-fucose, D-arabitol, L-arabitol, potassium gluconate, potassium 2-ketogluconate, potassium-5-ketogluconate.
Table 2.

Differential characteristics of Enorma timonensis GD5T, Enorma massiliensis strain phIT, Collinsella aerofaciens strain YIT 10235T, Collinsella tanakei strain YIT 12064T and Coriobacterium glomerans strain PW2.

Properties

E. timonensis

E. massiliensis

C. aerofaciens

C. tanakei

C. glomerans

Cell diameter (µm)

0.58

0.57

0.3–0.7

0.5

NA

Oxygen requirement

anaerobic

anaerobic

anaerobic

anaerobic

anaerobic

Gram stain

+

+

+

+

+

Salt requirement

na

na

na

na

na

Motility

na

Endospore formation

na

Production of

     

Alkaline phosphatase

+

na

Acid phosphatase

na

+

na

Catalase

na

na

Oxidase

na

na

Nitrate reductase

na

na

Urease

na

α-galactosidase

+

+

na

β-galactosidase

+

+

+

na

β-glucuronidase

+

na

α-glucosidase

+

+

+

na

β-glucosidase

+

+

+

na

Esterase

na

na

Esterase lipase

na

na

Indole

na

na

N-acetyl-β-glucosaminidase

na

6-Phospho-β-galactosidase

na

Arginine arylamidase

+

+

+

+

na

glutamic acid decarboxylase

na

Leucyl glycine arylamidase

+

+

na

Alanine arylamidase

na

Proline arylamidase

+

+

+

+

na

Serine arylamidase

+

na

Tyrosine arylamidase

na

Glycine arylamidase

+

+

na

Utilization of

     

D-mannose

+

+

+

+

Habitat

human gut

human gut

human gut

human gut

na

na: data not available

+/−: depending on tests used

E. timonensis is susceptible to amoxicillin-clavulanic acid, metronidazole, imipenem, vancomycin, rifampicin, gentamicin and resistant to penicillin G, amoxicillin, ceftriaxon, erythromycin, and trimethoprim/sulfamethoxazole.

Matrix-assisted laser-desorption/ionization time-of-flight (MALDI-TOF) MS protein analysis was carried out as previously described [55] using a Microflex spectrometer (Bruker Daltonics, Leipzig, Germany). Twelve distinct deposits were done for strain GD5T from twelve isolated colonies. The twelve GD5T spectra were imported into the MALDI BioTyper software (version 2.0, Bruker) and analyzed by standard pattern matching (with default parameter settings) against the main spectra of 4,706 bacteria, which were used as reference data, in the BioTyper database. For strain GD5T, no significant score was obtained, thus suggesting that our isolate was not a member of a known species. We added the spectrum from strain GD5T to our database (Figure 4, Figure 5).
Figure 4.

Reference mass spectrum from E. timonensis strain GD5T. Spectra from 12 individual colonies were compared and a reference spectrum was generated.

Figure 5.

Gel view comparing E. timonensis sp. nov strain GD5T and other members of the Coriobacteriaceae family. The gel view displays the raw spectra of all loaded spectrum files arranged in a pseudo-gel like look. The x-axis records the m/z value. The left y-axis displays the running spectrum number originating from subsequent spectra loading. The peak intensity is expressed by a gray scale scheme code. The color bar and the right y-axis indicate the relation between the color a peak is displayed with and the peak intensity in arbitrary units. Displayed species are indicated on the left.

Genome sequencing information

Genome project history

The organism was selected for sequencing on the basis of its phylogenetic position and 16S rDNA similarity to E. massiliensis and other members of the family Coriobacteriaceae and is part of a study of the human digestive flora aiming at isolating all bacterial species within human feces [13]. It was the 2nd genome of an Enorma species and the first genome of E. timonensis sp. nov. The GenBank accession number is CAPF00000000 and consists of 105 contigs. Table 3 shows the project information and its association with MIGS version 2.0 compliance [48].
Table 3.

Project information

MIGS ID

Property

Term

MIGS-31

Finishing quality

High-quality draft

MIGS-28

Libraries used

One paired-end 454 3-kb library

MIGS-29

Sequencing platforms

454 GS FLX Titanium

MIGS-31.2

Fold coverage

43.5

MIGS-30

Assemblers

Newbler version 2.5.3

MIGS-32

Gene calling method

Prodigal

 

INSDC ID

PRJEB543

 

GenBank ID

CAPF00000000

 

GenBank Date of Release

April 25, 2013

MIGS-13

Project relevance

Study of the human gut microbiome

Growth conditions and DNA isolation

Enorma timonesis sp. nov., strain GD5T (= CSUR P900 = DSM 26111), was grown anaerobically on 5% sheep blood-enriched Columbia agar (BioMerieux) at 37°C. Four Petri dishes were spread and resuspended in 1ml TE buffer prior to being treated with 2.5 µg/µL lysozyme for 30 minutes at 37°C, and then with Proteinase K overnight at 37°C. The DNA was then purified by 3 successive phenol-chloroform extractions followed by an ethanol precipitation at −20°C overnight. Following centrifugation, the DNA was then resuspended in 305 µL TE buffer. The DNA was then concentrated and purified using a QIAamp kit (Qiagen). The yield and concentration was measured by the Quant-it Picogreen kit (Invitrogen) on the Genios Tecan fluorometer at 66.5 ng/µl.

Genome sequencing and assembly

DNA (5 µg) was mechanically fragmented on a Hydroshear device (Digilab, Holliston, MA, USA) with an enrichment size at 3–4kb. The DNA fragmentation was visualized through the Agilent 2100 BioAnalyzer on a DNA labchip 7500 with an optimal size of 4.4kb. A 3kb paired-end library was constructed according to the 454 GS FLX Titanium paired-end protocol (Roche). Circularization and nebulization were performed and generated a pattern with an optimal at 470 bp. After PCR amplification through 17 cycles followed by double size selection, the single stranded paired-end library was then quantified on the Agilent 2100 BioAnalyzer on a RNA pico 6000 LabChip at 136 pg/µL. The library concentration equivalence was calculated as 5.31E+08 molecules/µL. The library was stored at −20°C until further use.

The paired-end library was clonally amplified with 0.5cpb and 2cbp in 2 SV-emPCR with the GS Titanium SV-emPCR Kit (Lib-L) v2 (Roche). The yields of the emPCRs were 9.37 and 14.09%, respectively, in the range of 5 to 20% from the Roche procedure.

Approximately 790,000 beads were loaded on 1/4 region of a GS Titanium PicoTiterPlate PTP Kit 70x75 and sequenced with the GS-FLX Titanium Sequencing Kit XLR70 (Roche). The run was performed overnight and then analyzed on the cluster through the gsRunBrowser and gsAssembler (Roche). A total of 282,633 passed filter wells were obtained and generated 102.68Mb with a length average of 363 bp. The globally passed filter sequences were assembled using Newbler with 90% identity and 40bp as overlap. The final assembly identified 5 scaffolds and 105 large contigs (>1,500 bp) generating a genome size of 2.36 Mb which corresponds to a coverage of 43.5 genome equivalents.

Genome annotation

Open Reading Frames (ORFs) were predicted using Prodigal [56] with default parameters. However, the predicted ORFs were excluded if they spanned a sequencing gap region. The predicted bacterial protein sequences were searched against the GenBank [57] and Clusters of Orthologous Groups (COG) databases using BLASTP. The tRNAs and rRNAs were predicted using the tRNAScanSE [58] and RNAmmer [59] tools, respectively. Lipoprotein signal peptides and numbers of transmembrane helices were predicted using SignalP [60] and TMHMM [61], respectively. ORFans were identified if their BLASTP E-value was lower than 1e-03 for alignment length greater than 80 amino acids. If alignment lengths were smaller than 80 amino acids, we used an E-value of 1e-05. Such parameter thresholds have already been used in previous works to define ORFans. Artemis [62] and DNA Plotter [63] were used for data management and visualization of genomic features, respectively. The Mauve alignment tool (version 2.3.1) was used for multiple genomic sequence alignment [64]. To estimate the mean level of nucleotide sequence similarity at the genome level between E. timonensis and five other members of the family Coriobacteriaceae (Table 6), we used the Average Genomic Identity Of gene Sequences (AGIOS) home-made software. Briefly, this software combines the Proteinortho software [65] for detecting orthologous proteins between genomes compared two by two, then retrieves the corresponding genes and determines the mean percentage of nucleotide sequence identity among orthologous ORFs using the Needleman-Wunsch global alignment algorithm. Enorma timonensis strain GD5T was compared to E. massiliensi strain phIT (GenBank accession number CAGZ00000000), C. aerofaciens strain ATCC 25986 (AAVN00000000), C. tanakei strain YIT 12063 (ADLS00000000) and C. glomerans strain PW2 (NC_015389).

Genome properties

The genome is 2,365,123 bp long (1 chromosome, no plasmid) with a 65.8% G+C content (Figure 6 and Table 4). Of the 2,060 predicted chromosomal genes, 2,006 were protein-coding genes and 52 were RNAs, including a complete rRNA operon, an additional 5S rRNA and 48 tRNAs. A total of 1,384 genes (67.18%) were assigned a putative function. Fifty-five genes were identified as ORFans (2.74%) and the remaining genes were annotated as hypothetical proteins. The properties and statistics of the genome are summarized in Tables 3 and 4. The distribution of genes into COGs functional categories is presented in Table 5.
Figure 6.

Graphical circular map of the chromosome. From the outside in: genes on the forward strand (colored by COG categories), genes on the reverse strand (colored by COG categories), RNA genes (rRNAs green, tRNAs red), GC skew (purple: negative values, olive: positive values), and G+C content plot.

Table 4.

Nucleotide content and gene count levels of the genome

Attribute

Value

% of totala

Genome size (bp)

2,365,123

 

DNA coding region (bp)

2,061,753

87.17

DNA G+C content (bp)

1,556,250

65.8

Total genes

2,060

100

RNA genes

52

2.62

Protein-coding genes

2,006

97.37

Genes with function prediction

1,384

67.18

Genes assigned to COGs

1,518

73.68

Genes with peptide signals

88

4.27

Genes with transmembrane helices

466

22.62

a The total is based on either the size of the genome in base pairs or the total number of protein coding genes in the annotated genome

Table 5.

Number of genes associated with the 25 general COG functional categories

Code

Value

% of totala

Description

J

140

6.98

Translation

A

0

0

RNA processing and modification

K

152

7.58

Transcription

L

90

4.48

Replication, recombination and repair

B

1

0.05

Chromatin structure and dynamics

D

20

1.0

Cell cycle control, mitosis and meiosis

Y

0

0

Nuclear structure

V

52

2.59

Defense mechanisms

T

61

3.04

Signal transduction mechanisms

M

92

4.58

Cell wall/membrane biogenesis

N

3

0.15

Cell motility

Z

0

0

Cytoskeleton

W

0

0

Extracellular structures

U

16

0.80

Intracellular trafficking and secretion

O

45

2.24

Posttranslational modification, protein turnover, chaperones

C

78

3.88

Energy production and conversion

G

224

11.16

Carbohydrate transport and metabolism

E

172

8.57

Amino acid transport and metabolism

F

50

2.49

Nucleotide transport and metabolism

H

40

1.99

Coenzyme transport and metabolism

I

38

1.89

Lipid transport and metabolism

P

71

3.54

Inorganic ion transport and metabolism

Q

13

0.65

Secondary metabolites biosynthesis, transport and catabolism

R

205

10.22

General function prediction only

S

118

5.88

Function unknown

-

488

24.32

Not in COGs

a The total is based on the total number of protein coding genes in the annotated genome

Genome comparison of E. timonensis with other members of the Coriobacteriaceae family

We compared the genome of E. timonensis strain GD5T with those of E. massiliensis phI, Collinsella aerofaciens strain ATCC 25986, Collinsella tanakaei strain YIT 12063 and Coriobacterium glomerans strain PW2 (Table 6).
Table 6.

Genomic comparison of E. timonensis and four other members of the Coriobacteriaceae family.

Species

Strain

Genome accession number

Genome size (Mb)

G+C content

E. timonensis

GD5T

CAPF00000000

2,365,123

65.80

E. massiliensis

phI

CAGZ01000000

2,263,008

62.0

C. aerofaciens

ATCC 25986

AAVN00000000

2,439,869

60.54

C. glomerans

PW2

NC_015389

2,115,681

60.40

C. tanakaei

YIT 12063

ADLS00000000

2,482,197

60.23

The draft genome sequence of E. timonensis strain GD5T is smaller than those of C. aerofaciens and C. tanakaei (2.36, 2.43 and 2.48 Mb, respectively), but larger than those of E. massiliensis and C. glomerans (2.26 and 2.11 Mb, respectively). The G+C content of E. timonensis is larger than those of E. massiliensis, C. aerofaciens, C. tanakaei and C. glomerans (65.80, 62.0, 60.54, 60.23 and 60.40%, respectively). The gene content of E. timonensis is smaller to those of E. massiliensis, C. glomerans and C. tanakaei (2,006, 2,159 and 2,195, respectively) but larger than those of C. aerofaciens and C. tanakaei (1,901 and 1,768, respectively). The distribution of genes into COG categories was not entirely similar in all compared genomes (Figure 7).
Figure 7.

Distribution of functional classes of predicted genes in the E. timonensis (colored in light blue), E. massiliensis (dark blue), Coriobacterium glomerans (green), Colinsella aerofaciens (yellow) and Colinsella tanakaei (red) chromosomes, according to the clusters of orthologous groups of proteins.

In addition, E. timonensis shared 1,109, 1,026, 880 and 1,077 orthologous genes with E. massiliensis, C. aerofaciens, C. glomerans and C. tanakaei respectively. The average genomic nucleotide sequence identity ranged from 66.37 to 79.44% among Coriobacteriaceae family members, and from 66.01 to 79.44% between E. timonensis and other species (Table 6 and Table 7).
Table 7.

Genomic comparison of E. timonensis and four other members of the Coriobacteriaceae family.

 

E. timonensis

E. massiliensis

C. aerofaciens

C. glomerans

C. tanakaei

E. timonensis

2,006

1109

1026

880

1077

E. massiliensis

79.44

1,901

1046

899

1103

C. aerofaciens

66.37

66.01

2,159

880

1062

C. glomerans

73.39

72.38

66.15

1,768

913

C. tanakaei

74.02

73.43

64.96

71.27

2,195

aUpper right triangle: numbers of orthologous protein shared between genomes; lower left triangle: average percentage similarity of nucleotides corresponding to orthologous proteins shared between genomes; bold face: numbers of proteins per genome.

Conclusion

On the basis of phenotypic, phylogenetic and genomic analyses (taxono-genomics), we formally propose the creation of Enorma timonensis sp. nov. that contains strain GD5T. This bacterium has been found in France.

Description of Enorma timonensis sp. nov.

Enorma timonensis (ti.mo.nen’sis. L. gen. masc. timonensis, of Timone, the name of the hospital where strain GD5T was cultivated). Colonies are translucent grey and 0.4 mm in diameter on blood-enriched Columbia agar. Cells are rod-shaped with a mean diameter of 0.58 µm and a mean length of 1.32 µm. Optimal growth is achieved in anaerobic conditions. No growth is observed in aerobic or microaerophilic conditions. Growth occurs between 37–45°C, with optimal growth being observed at 37°C on blood-enriched Columbia agar. Cells are Gram-positive, non-endospore forming, and non-motile. Cells are negative for catalase and oxidase. Using an API ZYM strip, positive reactions are observed for leucine arylamidase, valine arylamidase, cystin arylamidase, naphthol-AS-BI-phosphohydrolase, β-galactosidase, β-glucuronidase, α-glucosidase and β-glucosidase. Negative reactions are observed for acid phosphatase, nitrate reduction, urease alkaline phosphatase, esterase (C4), esterase lipase (C8), lipase (C14), trypsin, α-chemotrypsin, acid phosphatase, α-galactosidase, N-actetyl-β-glucosaminidase, α-mannosidase, α-fucosidase. Using an API Rapid ID 32A strip, positive reactions are observed for proline arylamidase, phenylalanine arylamidase, histidin arylamidase, serine arylamidase. Negative reactions are observed for urease, arginine dihydrolase, tyrosin arylamidase, leucyl-glycyl arylamidase, alanine arylamidase, glycine arylamidase and arginine arylamidase. Using an API 50 CH strip, fermentation or assimilation was not observed.

Cells are susceptible to amoxicillin-clavulanic acid, metronidazole, imipenem, vancomycin, rifampicin, gentamicin and resistant to penicillin G, amoxicillin, ceftriaxon, erythromycin, and trimethoprim/sulfamethoxazole. The 16S rDNA and genome sequences are deposited in GenBank under accession numbers JX424767 and CAPF00000000, respectively. The G+C content of the genome is 65.8%. The habitat of the organism is the human digestive tract. The type strain GD5T (= CSUR P900 = DSM 26111) was isolated from the fecal flora of a 53-year old French patient hospitalized in an intensive care unit. This strain has been found in Marseille, France.

Notes

Declarations

Acknowledgements

The authors thank the Xegen Company for automating the genomic annotation process. This study was funded by the Mediterranee Infection Foundation.

Authors’ Affiliations

(1)
Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, Institut Hospitalo-Universitaire Méditerranée-Infection, Faculté de médecine, Aix-Marseille Université
(2)
Service de Réanimation Médicale, Hôpital Nord
(3)
Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University

References

  1. Lagier JC, Armougom F, Million M, Hugon P, Pagnier I, Robert C, Bittar F, Fournous G, Gimenez G, Maraninchi M, et al. Microbial culturomics: paradigm shift in the human gut microbiome study. Clin Microbiol Infect 2012; 18:1185–1193. PubMedView ArticlePubMedGoogle Scholar
  2. Dubourg G, Lagier JC, Armougom F, Robert C, Hamad I, Brouqui P. The gut microbiota of a patient with resistant tuberculosis is more comprehensively studied by culturomics than by metagenomics. Eur J Clin Microbiol Infect Dis 2013; 32:637–645. PubMed http://dx.doi.org/10.1007/s10096-012-1787-3View ArticlePubMedGoogle Scholar
  3. Pfleiderer A, Lagier JC, Armougom F, Robert C, Vialettes B, Raoult D. Culturomics identified 11 new bacterial species from a single anorexia nervosa stool sample. [Epub ahead of print]. Eur J Clin Microbiol Infect Dis 2013.
  4. Whitman WB, Coleman DC, Wiebe WJ. Prokaryotes: the unseen majority. Proc Natl Acad Sci USA 1998; 95:6578–6583. PubMed http://dx.doi.org/10.1073/pnas.95.12.6578PubMed CentralView ArticlePubMedGoogle Scholar
  5. Qin J, Li R, Raes J, Arumugam M, Burgdorf KS, Manichanh C, Nielsen T, Pons N, Levenez F, Yamada T, et al. A human gut microbial gene catalogue established by metagenomic sequencing. Nature 2010; 464:59–65. PubMed http://dx.doi.org/10.1038/nature08821PubMed CentralView ArticlePubMedGoogle Scholar
  6. Clemente JC, Ursell LK, Parfrey LW, Knight R. The impact of the gut microbiota on human health: an integrative view. Cell 2012; 148:1258–1270. PubMed http://dx.doi.org/10.1016/j.cell.2012.01.035View ArticlePubMedGoogle Scholar
  7. Tindall BJ, Rossello-Mora R, Busse HJ, Ludwig W, Kampfer P. Notes on the characterization of prokaryote strains for taxonomic purposes. Int J Syst Evol Microbiol 2010; 60:249–266. PubMed http://dx.doi.org/10.1099/ijs.0.016949-0View ArticlePubMedGoogle Scholar
  8. Stackebrandt E, Ebers J. Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 2006; 33:152–155.Google Scholar
  9. Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandier O, Krichevsky MI, Moore LH, Moore WEC, Murray RGE, Stackebrandt E, et al. Report of the ad hoc committee on reconciliation of approaches to bacterial systematic. Int J Syst Bacterid 1987; 37:463–464. http://dx.doi.org/10.1099/00207713-37-4-463View ArticleGoogle Scholar
  10. Rossello-Mora R. DNA-DNA Reassociation Methods Applied to Microbial Taxonomy and Their Critical Evaluation. In: Stackebrandt E (ed), Molecular Identification, Systematics, and population Structure of Prokaryotes. Springer, Berlin, 2006; p. 23–50.View ArticleGoogle Scholar
  11. Welker M, Moore ER. Applications of whole-cell matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry in systematic microbiology. Syst Appl Microbiol 2011; 34:2–11. PubMed http://dx.doi.org/10.1016/j.syapm.2010.11.013View ArticlePubMedGoogle Scholar
  12. Kokcha S, Mishra AK, Lagier JC, Million M, Leroy Q, Raoult D, Fournier PE. Non-contiguous finished genome sequence and description of Bacillus timonensis sp. nov. Stand Genomic Sci 2012; 6:346–355. PubMed http://dx.doi.org/10.4056/sigs.2776064PubMed CentralView ArticlePubMedGoogle Scholar
  13. Lagier JC, El Karkouri K, Nguyen TT, Armougom F, Raoult D, Fournier PE. Non-contiguous finished genome sequence and description of Anaerococcus senegalensis sp. nov. Stand Genomic Sci 2012; 6:116–125. PubMed http://dx.doi.org/10.4056/sigs.2415480PubMed CentralView ArticlePubMedGoogle Scholar
  14. Mishra AK, Gimenez G, Lagier JC, Robert C, Raoult D, Fournier PE. Non-contiguous finished genome sequence and description of Alistipes senegalensis sp. nov. Stand Genomic Sci 2012; 6:304–314. http://dx.doi.org/10.4056/sigs.2625821View ArticleGoogle Scholar
  15. Lagier JC, Armougom F, Mishra AK, Ngyuen TT, Raoult D, Fournier PE. Non-contiguous finished genome sequence and description of Alistipes timonensis sp. nov. Stand Genomic Sci 2012; 6:315–324. PubMed http://dx.doi.org/10.4056/sigs.2685971PubMed CentralView ArticlePubMedGoogle Scholar
  16. Mishra AK, Lagier JC, Robert C, Raoult D, Fournier PE. Non-contiguous finished genome sequence and description of Clostridium senegalense sp. nov. Stand Genomic Sci 2012; 6:386–395. PubMed http://dx.doi.org/10.4056/sigs.2766062PubMed CentralPubMedGoogle Scholar
  17. Mishra AK, Lagier JC, Robert C, Raoult D, Fournier PE. Non-contiguous finished genome sequence and description of Peptoniphilus timonensis sp. nov. Stand Genomic Sci 2012; 7:1–11. PubMed http://dx.doi.org/10.4056/sigs.2956294PubMed CentralView ArticlePubMedGoogle Scholar
  18. Mishra AK, Lagier JC, Rivet R, Raoult D, Fournier PE. Non-contiguous finished genome sequence and description of Paenibacillus senegalensis sp. nov. Stand Genomic Sci 2012; 7:70–81. PubMed http://dx.doi.org/10.4056/sigs.3056450PubMed CentralView ArticlePubMedGoogle Scholar
  19. Lagier JC, Gimenez G, Robert C, Raoult D, Fournier PE. Non-contiguous finished genome sequence and description of Herbaspirillum massiliense sp. nov. Stand Genomic Sci 2012; 7:200–209. PubMed http://dx.doi.org/10.4056/sigs.3086474PubMed CentralPubMedGoogle Scholar
  20. Kokcha S, Ramasamy D, Lagier JC, Robert C, Raoult D, Fournier PE. Non-contiguous finished genome sequence and description of Brevibacterium senegalense sp. nov. Stand Genomic Sci 2012; 7:233–245. PubMed http://dx.doi.org/10.4056/sigs.3256677PubMed CentralView ArticlePubMedGoogle Scholar
  21. Ramasamy D, Kokcha S, Lagier JC, N’Guyen TT, Raoult D, Fournier PE. Non-contiguous finished genome sequence and description of Aeromicrobium massilense sp. nov. Stand Genomic Sci 2012; 7:246–257. PubMed http://dx.doi.org/10.4056/sigs.3306717PubMed CentralView ArticlePubMedGoogle Scholar
  22. Lagier JC, Ramasamy D, Rivet R, Raoult D, Fournier PE. Non-contiguous finished genome sequence and description of Cellulomonas massiliensis sp. nov. Stand Genomic Sci 2012; 7:258–270. PubMed http://dx.doi.org/10.4056/sigs.3316719PubMed CentralView ArticlePubMedGoogle Scholar
  23. Lagier JC, Karkouri K, Rivet R, Couderc C, Raoult D, Fournier PE. Non contiguous-finished genome sequence and description of Senegalemassilia anaerobia gen. nov., sp. nov. Stand Genomic Sci 2013; 7:343–356. PubMed http://dx.doi.org/10.4056/sigs.3246665PubMed CentralView ArticlePubMedGoogle Scholar
  24. Mishra AK, Hugon P, Nguyen TT, Robert C, Couderc C, Raoult D, Fournier PE. Non contiguous-finished genome sequence and description of Peptoniphilus obesi sp. nov. Stand Genomic Sci 2013; 7:357–369. PubMed http://dx.doi.org/10.4056/sigs.32766871PubMed CentralView ArticlePubMedGoogle Scholar
  25. Mishra AK, Lagier JC, Nguyen TT, Raoult D, Fournier PE. Non contiguous-finished genome sequence and description of Peptoniphilus senegalensis sp. nov. Stand Genomic Sci 2013; 7:370–381. PubMed http://dx.doi.org/10.4056/sigs.3366764PubMed CentralView ArticlePubMedGoogle Scholar
  26. Lagier JC, Karkouri K, Mishra AK, Robert C, Raoult D, Fournier PE. Non contiguous-finished genome sequence and description of Enterobacter massiliensis sp. nov. Stand Genomic Sci 2013; 7:399–412. PubMed http://dx.doi.org/10.4056/sigs.3396830PubMed CentralView ArticlePubMedGoogle Scholar
  27. Hugon P, Ramasamy D, Rivet R, Raoult D, Fournier PE. Non contiguous-finished genome sequence and description of Alistipes obesi sp. nov. Stand Genomic Sci 2013; 7:427–439. PubMed http://dx.doi.org/10.4056/sigs.3336746PubMed CentralView ArticlePubMedGoogle Scholar
  28. Hugon P, Mishra AK, Nguyen TT, Raoult D, Fournier PE. Non-contiguous finished genome sequence and description of Brevibacillus massiliensis sp. nov. Stand Genomic Sci 2013; 8:1–14. PubMed http://dx.doi.org/10.4056/sigs.3466975PubMed CentralView ArticlePubMedGoogle Scholar
  29. Mishra AK, Hugon P, Nguyen TT, Raoult D, Fournier PE. Non contiguous-finished genome sequence and description of Enorma massiliensis gen. nov., sp. nov., a new member of the Family Coriobacteriaceae. Stand Genomic Sci 2013; 8:290–305. PubMed http://dx.doi.org/10.4056/sigs.3426906PubMed CentralView ArticlePubMedGoogle Scholar
  30. Ramasamy D, Lagier JC, Gorlas A, Raoult D, Fournier PE. Non contiguous-finished genome sequence and description of Bacillus massiliosenegalensis sp. nov. Stand Genomic Sci 2013; 8:264–278. PubMed http://dx.doi.org/10.4056/sigs.3496989PubMed CentralView ArticlePubMedGoogle Scholar
  31. Ramasamy D, Lagier JC, Nguyen TT, Raoult D, Fournier PE. Non contiguous-finished genome sequence and description of Dielma fastidiosa gen. nov., sp. nov., a new member of the Family Erysipelotrichaceae. Stand Genomic Sci 2013; 8:336–351. PubMed http://dx.doi.org/10.4056/sigs.3567059PubMed CentralView ArticlePubMedGoogle Scholar
  32. Mishra AK, Pfleiderer A, Lagier JC, Robert C, Raoult D, Fournier PE. Non contiguous-finished genome sequence and description of Bacillus massilioanorexicus sp. nov. Stand Genomic Sci 2013; 8:465–479. http://dx.doi.org/10.4056/sigs.4087826PubMed CentralView ArticlePubMedGoogle Scholar
  33. Hugon P, Ramasamy D, Robert C, Couderc C, Raoult D, Fournier PE. Non-contiguous finished genome sequence and description of Kallipyga massiliensis gen. nov., sp. nov., a new member of the family Clostridiales Incertae Sedis XI. Stand Genomic Sci 2013; 8:500–515. http://dx.doi.org/10.4056/sigs.4047997PubMed CentralView ArticlePubMedGoogle Scholar
  34. Padhmanabhan R, Lagier JC, Dangui NPM, Michelle C, Couderc C, Raoult D, Fournier PE. Non-contiguous finished genome sequence and description of Megasphaera massiliensis. Stand Genomic Sci 2013; 8:525–538. http://dx.doi.org/10.4056/sigs.4077819View ArticleGoogle Scholar
  35. Stackebrandt E, Rainey FA, Ward-Rainey NL. Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 1997; 47:479–491. http://dx.doi.org/10.1099/00207713-47-2-479View ArticleGoogle Scholar
  36. List of Prokaryotic names with standing nomenclature (LPSN). http://www.bacterio.cict.fr.
  37. Maruo T, Sakamoto M, Ito C, Toda T, Benno Y. Adlercreutzia equolifaciens gen. nov., sp. nov., an equol-producing bacterium isolated from human faeces, and emended description of the genus Eggerthella. Int J Syst Evol Microbiol 2008; 58:1221–1227. PubMed http://dx.doi.org/10.1099/ijs.0.65404-0View ArticlePubMedGoogle Scholar
  38. Minamida K, Ota K, Nishimukai M, Tanaka M, Abe A, Sone T, Tomita F, Hara H, Asano K. Asaccharobacter celatus gen. nov., sp. nov., isolated from rat caecum. Int J Syst Evol Microbiol 2008; 58:1238–1240. PubMed http://dx.doi.org/10.1099/ijs.0.64894-0View ArticlePubMedGoogle Scholar
  39. Collins MD, Wallbanks S. Comparative sequence analyses of the 16S rRNA genes of Lactobacillus minutus, Lactobacillus rimae and Streptococcus parvulus: proposal for the creation of a new genus Atopobium. FEMS Microbiol Lett 1992; 74:235–240. PubMed http://dx.doi.org/10.1111/j.1574-6968.1992.tb05372.xPubMedGoogle Scholar
  40. Kageyama A, Benno Y, Nakase K. Phylogenetic and phenotypic evidence for the transfer of Eubacterium aerofaciens to the genus Collinsella as Collinsella aerofaciens gen. nov., comb. nov. Int J Syst Bacteriol 1999; 49:557–565. PubMed http://dx.doi.org/10.1099/00207713-49-2-557View ArticlePubMedGoogle Scholar
  41. Haas F, König H. Coriobacterium glomerans gen. nov., sp. nov. from the intestinal tract of the red soldier bug. Int J Syst Bacteriol 1988; 38:382–384. http://dx.doi.org/10.1099/00207713-38-4-382View ArticleGoogle Scholar
  42. Nakazawa F, Poco SE, Ikeda T, Sato M, Kalfas S, Sundqvist G, Hoshino E. Cryptobacterium curtam gen. nov., sp. nov., a new genus of gram-positive anaerobic rod isolated from human oral cavities. Int J Syst Bacteriol 1999; 49:1193–1200. PubMed http://dx.doi.org/10.1099/00207713-49-3-1193View ArticlePubMedGoogle Scholar
  43. Anderson RC, Rasmussen MA, Jensen NS, Allison MJ. Denitrobacterium detoxificans gen. nov., sp. nov., a ruminai bacterium that respires on nitrocompounds. Int J Syst Evol Microbiol 2000; 50:633–638. PubMed http://dx.doi.org/10.1099/00207713-50-2-633View ArticlePubMedGoogle Scholar
  44. Wade WG, Downes J, Dymock D, Hiom S, Weightman AJ, Dewhirst FE, Paster BJ, Tzellas N, Coleman B. The family Coriobacteriaceae: reclassification of Eubacterium exiguum (Poco et al. 1996) and Peptostreptococcus heliotrinreducens (Lanigan 1976) as Slackia exigua gen. nov., comb. nov. and Slackia heliotrinireducens gen. nov., comb, nov., and Eubacterium lentum (Prevot 1938) as Eggerthella lenta gen. nov., comb. nov. Int J Syst Bacteriol 1999; 49:595–600. PubMed http://dx.doi.org/10.1099/00207713-49-2-595View ArticlePubMedGoogle Scholar
  45. Clavel T, Charrier C, Braune A, Wenning M, Blaut M, Haller D. Isolation of bacteria from the ileal mucosa of TNFdeltaARE mice and description of Enterorhabdus mucosicola gen. nov., sp. nov. Int J Syst Evol Microbiol 2009; 59:1805–1812. PubMed http://dx.doi.org/10.1099/ijs.0.003087-0View ArticlePubMedGoogle Scholar
  46. Würdemann D, Tindall BJ, Pukall R, Lunsdorf H, Strompl C, Namuth T, Nahrstedt H, Wos-Oxley M, Ott S, Schreiber S, Timmis KN, Oxley AP. Gordonibacter pamelaeae gen. nov., sp. nov., a new member of the Coriobacteriaceae isolated from a patient with Crohn’s disease, and reclassification of Eggerthella hongkongensis Lau et al. 2006 as Paraeggerthella hongkongensis gen. nov., comb. nov. Int J Syst Evol Microbiol 2009; 59:1405–1415. PubMed http://dx.doi.org/10.1099/ijs.0.005900-0View ArticlePubMedGoogle Scholar
  47. Dewhirst FE, Paster BJ, Tzellas N, Coleman B, Downes J, Spratt DA, Wade WG. Characterization of novel human oral isolates and cloned 16S rDNA sequences that fall in the family Coriobacteriaceae: description of Olsenella gen. nov., reclassification of Lactobacillus uli as Olsenella uli comb. nov. and description of Olsenella profusa sp. nov. Int J Syst Evol Microbiol 2001; 51:1797–1804. PubMed http://dx.doi.org/10.1099/00207713-51-5-1797View ArticlePubMedGoogle Scholar
  48. Field D, Garrity G, Gray T, Morrison N, Selengut J, Sterk P, Tatusova T, Thomson N, Allen MJ, Angiuoli SV, et al. The minimum information about a genome sequence (MIGS) specification. Nat Biotechnol 2008; 26:541–547. PubMed http://dx.doi.org/10.1038/nbt1360PubMed CentralView ArticlePubMedGoogle Scholar
  49. Woese CR, Kandier O, Wheelis ML. Towards a natural system of organisms: proposal for the domains Archae, Bacteria, and Eukarya. Proc Natl Acad Sci USA 1990; 87:4576–4579. PubMed http://dx.doi.org/10.1073/pnas.87.12.4576PubMed CentralView ArticlePubMedGoogle Scholar
  50. Garrity GM, Holt JG. The Road Map to the Manual. In: Garrity GM, Boone DR, Castenholz RW (eds), Bergey’s Manual of Systematic Bacteriology, Second Edition, Volume 1, Springer, New York, 2001, p. 119–169.View ArticleGoogle Scholar
  51. Garrity GM, Holt JG. Taxonomic outline of the Archae and Bacteria. In: Garrity GM, Boone DR, Castenholz RW (eds), Bergey’s Manual of Systematic Bacteriology, second edition, Volume, Springer-Verlag, New York, 2001, p. 155–166.Google Scholar
  52. Gupta RS, Chen WJ, Adeolu M, Chai Y. Molecular signatures for the class Coriobacteriia and its different clades; proposal for division of the class Coriobacteriia into the emended order Coriobacteriales, containing the emended family Coriobacteriaceae and Atopobiaceae fam. nov., and Eggerthellales ord. nov., containing the family Eggerthellaceae fam. nov. Int J Syst Evol Microbiol 2013; 63:3379–3397. PubMed http://dx.doi.org/10.1099/ijs.0.048371-0View ArticlePubMedGoogle Scholar
  53. Zhi XY, Li WJ, Stackebrandt E. An update of the structure and 16S rRNA gene sequence-based definition of higher ranks of the class Actinobacteria, with the proposal of two new suborders and four new families and emended descriptions of the existing higher taxa. Int J Syst Evol Microbiol 2009; 59:589–608. PubMed http://dx.doi.org/10.1099/ijs.0.65780-0View ArticlePubMedGoogle Scholar
  54. Ashburner M, Ball CA, Blake JA, Botstein D, Butler H, Cherry JM, Davis AP, Dolinski K, Dwight SS, Eppig JT, et al. Gene ontology: tool for the unification of biology. The Gene Ontology Consortium. Nat Genet 2000; 25:25–29. PubMed http://dx.doi.org/10.1038/75556PubMed CentralView ArticlePubMedGoogle Scholar
  55. Seng P, Drancourt M, Gouriet F, La SB, Fournier PE, Rolain JM, Raoult D. Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Clin Infect Dis 2009; 49:543–551. PubMed http://dx.doi.org/10.1086/600885View ArticlePubMedGoogle Scholar
  56. Prodigal, http://prodigal.ornl.gov/
  57. Benson DA, Karsch-Mizrachi I, Clark K, Lipman DJ, Ostell J, Sayers EW. GenBank. Nucleic Acids Res 2012; 40:D48–D53. PubMed http://dx.doi.org/10.1093/nar/gkr1202PubMed CentralView ArticlePubMedGoogle Scholar
  58. Lowe TM, Eddy SR. tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence. Nucleic Acids Res 1997; 25:955–964. PubMedPubMed CentralView ArticlePubMedGoogle Scholar
  59. Lagesen K, Hallin P, Rodland EA, Staerfeldt HH, Rognes T, Ussery DW. RNAmmer: consistent and rapid annotation of ribosomal RNA genes. Nucleic Acids Res 2007; 35:3100–3108. PubMed http://dx.doi.org/10.1093/nar/gkm160PubMed CentralView ArticlePubMedGoogle Scholar
  60. Bendtsen JD, Nielsen H, von Heijne G, Brunak S. Improved prediction of signal peptides: SignalP 3.0. J Mol Biol 2004; 340:783–795. PubMed http://dx.doi.org/10.1016/j.jmb.2004.05.028View ArticlePubMedGoogle Scholar
  61. Krogh A, Larsson B, von Heijne G, Sonnhammer EL. Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes. J Mol Biol 2001; 305:567–580. PubMed http://dx.doi.org/10.1006/jmbi.2000.4315View ArticlePubMedGoogle Scholar
  62. Rutherford K, Parkhill J, Crook J, Horsnell T, Rice P, Rajandream MA, Barrell B. Artemis: sequence visualization and annotation. Bioinformatics 2000; 16:944–945. PubMed http://dx.doi.org/10.1093/bioinformatics/16.10.944View ArticlePubMedGoogle Scholar
  63. Carver T, Thomson N, Bleasby A, Berriman M, Parkhill J. DNAPlotter: circular and linear interactive genome visualization. Bioinformatics 2009; 25:119–120. PubMed http://dx.doi.org/10.1093/bioinformatics/btn578PubMed CentralView ArticlePubMedGoogle Scholar
  64. Darling AC, Mau B, Blattner FR, Perna NT. Mauve: multiple alignment of conserved genomic sequence with rearrangements. Genome Res 2004; 14:1394–1403. PubMed http://dx.doi.org/10.1101/gr.2289704PubMed CentralView ArticlePubMedGoogle Scholar
  65. Lechner M, Findeib S, Steiner L, Marz M, Stadler PF, Prohaska SJ. Proteinortho: Detection of (Co-)orthologs in large-scale analysis. BMC Bioinformatics 2011; 12:124. PubMed http://dx.doi.org/10.1186/1471-2105-12-124PubMed CentralView ArticlePubMedGoogle Scholar

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