Open Access

Genome sequence of a high agarase-producing strain Flammeovirga sp. SJP92

Standards in Genomic Sciences201712:13

https://doi.org/10.1186/s40793-017-0221-y

Received: 18 March 2016

Accepted: 7 December 2016

Published: 26 January 2017

Abstract

Flammeovirga sp. SJP92 is a Gram-negative, aerobic, rod-shaped, non-motile and non-flagellated strain that belongs to the family Flammeovirgaceae of the class Cytophagia. The strain was isolated from the intestine of abalone, which produces many extracellular agarases and exhibits efficient degradation activities on various polysaccharides, especially agarose. Here we present the high-quality draft genome of Flammeovirga sp. SJP92, together with its phenotypic characteristics. The genome sequence is 8, 534, 834 bp, which comprised with one chromosome and no plasmid. It contained 6, 291 protein-coding and 99 RNA genes, including 93 tRNA, 5 rRNA and 1 ncRNA genes.

Keywords

Flammeovirga Genome High agarase-producing

Introduction

Flammeovirga is one of genera belonging to the family Flammeovirgaceae of the class Cytophagia . There are five species have been reported in this genus, including F. aprica [1], F. arenaria , F. yaeyamensis [2], F. kamogawensis [3] and F. pacifica [4]. They are all marine bacterium and have a potent ability to degrade marine complex polysaccharides, such as agar, carrageenan [3, 58]. Among them, only two draft genome sequences have been published [9], namely Flammeovirga sp. OC4 (NZ_JTAM01000001.1) [5] and F. pacifica WPAGA1T (=CCTCC AB 2010364T=LMG 26175T=DSM 24597T=MCCC 1A06425T) [7].

Flammeovirga sp. SJP92 with high-producing agarase was isolated and identified from the intestine of abalone in Xiamen, China. It is closely related with Flammeovirga sp. NBRC 100896 (AB681288.1) and shared 99% similarities of 16S rRNA. In order to provide more genome information of Flammeovirga species and realize the function of Flammeovirga sp. SJP92 when degradingmarine complex polysaccharides, the genome of Flammeovirga sp. SJP92 was sequenced. In this study, we summarized its genomic characteristics, as well as general phenotypic properties. Other species of Flammeovirga genus were also compared with Flammeovirga sp. SJP92 in both phenotypic and genomic aspects.

Organism information

Classification and features

Flammeovirga sp. SJP92 was isolated from the digestion guts of abalone with high agar-degrading ability, and deposited in China General Microbiological Culture Collection Center (CGMCC 10071). Based on the phylogenetic tree constructed with 16S rRNA, Flammeovirga sp. SJP92 is closely related with Flammeovirga sp. NBRC 100896 (AB681288.1) (Fig. 1). It is Gram-negative, curved-rods (0.75 μm wide and 11–13 μm long) after growth on 2216E plate for 3 days at 30 °C. It is aerobic and not motile without any flagella (Fig. 2). Also it is able to utilize a relatively wide spectrum of carbon substrates for growth, including agar, starch, carrageenan, L-fructose, Tween40, Tween80, galactose, lactose and so on, but it cannot utilize cellulose. Its growth temperature ranges from 15 to 40 °C with optimum between 25 and 30 °C. In addition, the optimum salinities for the growth of Flammeovirga sp. SJP92 were 2 ~ 4% (Table 1). When compared with other Flammeovirga species, this strain is different from F. pacifica WPAGA1T [8] and F. aprica NBRC 15941 T [2] in catalase, urease and esterase lipase and in the utilization of starch, D-Mannitol, L-fructose, Tween40&80 and D-xylose, differences were also observed in growth temperature range (Table 2).
Fig. 1

Phylogenetic tree highlighting the position of Flammeovirga sp. SJP92 relative to other type and non-type strains with finished or non-contiguous finished genome sequences within the family Flammeovirga. Accession numbers of 16S rRNA gene sequences are indicated in brackets. Sequences were aligned using ClustalX [14] and a neighbor-joining tree obtained using the maximum-likelihood method within the MEGA version4.0 [20]. Numbers adjacent to the branches represent percentage bootstrap values based on 1000 replicates

Fig. 2

Transmission electron micrograph of Flammeovirga sp. SJP92, using a JEM-100CX at an operating voltage of 120 KV. The scale bar represents 2 μm

Table 1

Classification and general features of Flammeovirga sp.SJP92

MIGS ID

Property

Term

Evidence Codea

 

Current classification

Domain Bacteria

TAS [21]

 

Phylum Bacteroidetes

TAS [22]

 

Class Cytophagia

TAS [23, 24]

 

Order Cytophagales

TAS [25, 26]

 

Family Flammeovirgaceae

TAS [27]

 

Genus Flammeovirga

TAS [1]

 

Species Flammeovirga sp.

TAS [5, 7]

  

Strain SJP92

IDA

 

Gram Stain

Negative

IDA

 

Cell shape

Curved-rods

IDA

 

Motility

None

IDA

 

Sporulation

Non-sporulating

IDA

 

Temperature range

15 ~ 40 °C

IDA

 

Optimum temperature

25 ~ 30 °C

IDA

 

pH range; Optimum

5 ~ 9, 8

IDA

 

Carbon source

Agar, Starch, Carrageenan, D-galactose, L-fructose, Tween40&80

IDA

MIGS-6

Habitat

Intestinal tract

IDA

MIGS-6.3

Salinity

0.5–8% NaCl (w/v)

IDA

MIGS-22

Oxygen

Aerobic

IDA

MIGS-15

Biotic relationship

Free-living

IDA

MIGS-14

Pathogenicity

Unknown

NAS

MIGS-4

Geographic location

Xamen city, China

IDA

MIGS-5

Sample collection

October 2006

IDA

MIGS-4.1

Latitude

24°26'

IDA

MIGS-4.2

Longitude

118°04'

IDA

MIGS-4.4

Altitude

Sea level

IDA

aEvidence codes: IDA Inferred from Direct Assay, TAS Traceable Author Statement (i.e., a direct report exists in the literature), NAS Non-traceable Author Statement (i.e., not directly observed for the living, isolated sample, but based on a generally accepted property for the species, or anecdotal evidence). These evidence codes are from the Gene Ontology project [28]. If the evidence code is IDA, then the property should have been directly observed for a live isolate by one of the authors, or an expert or reputable institution mentioned in the acknowledgement

Table 2

Differential phenotypic characteristics between Flammeovirga sp. SJP92 and other Flammeovirga species

Characteristic

1

2

3

Cell diameter (um)

11 ~ 13 × 0.75

3.0 ~ 8.0 × 0.5 ~ 0.8

1.7 ~ 96 × 0.5 ~ 0.9

Salinity/Optimum(w/v)

0.5 ~ 8%/2 ~ 4%

0–5%/3%

1–5%/3%

Temperature range (°C)

15 ~ 40

4–42

15–30

Number of polar flagella

None

None

None

Production of

 Agarase

+

+

+

 Catalase

+

 Oxidase

+

+

+

 Esterase lipase

_

±

±

 Urease

+

 β-Galactosidase

+

±

ND

 α-Galactosidase

+

+

ND

 Nitrate reductase

+

+

+

 Alkaline/Acid phosphatase

+

+

+

 Carbon source

 Gelatin

ND

 Agar

+

+

+

 Starch

+

 Cellulose

 D-galactose

+

+

+

 D-Mannitol

±

 L-fructose

+

+

 Tween40&80

+

 D-xylose

-

+

+

 Geographic location

XiaMen, China

157 °249′ 310″ E 19° 309′ 300″ N

Iriomote/Ishigaki Islands

 Habitat

Intestinal tract

Deep-sea sediment

Seaweeds/coastal sands/dead leaves

Strains: 1, Flammeovirga sp. SJP92; 2, F. pacifica WPAGA1T; 3, F. aprica NBRC15941T.+: positive result, −: negative result, ±:weak positive result, ND no data available

Genome sequencing information

Genome project history

This organism was initially selected for sequencing on the basis of its high agar-degrading ability. Sequencing of the Flammeovirga sp. SJP92 genome was performed at the Beijing Novogene Bioinformatics Technology Co., Ltd. The Whole Genome Shotgun project has been deposited at the DDBJ/EMBL/GenBank database under the accession number LQAQ00000000. The project information and its association with MIGS version 2.0 compliance were presented in Table 3 [9].
Table 3

Genome sequencing project information for Flammeovirga sp. SJP92

MIGS ID

Property

Term

MIGS-31

Finishing quality

High-quality draft

MIGS-28

Libraries used

500 bp pair-end&5 kb mate-end libraries

MIGS-29

Sequencing platforms

Illumina HiSeq2500,

MIGS-31.2

Fold coverage

215×

MIGS-30

Assemblers

SOAPdenovo v.2.04

MIGS-32

Gene calling method

NCBI PGAP pipeline

 

Locus Tag

AVL50

 

GenBank ID

LQAQ00000000

 

GenBank Date of Release

March 9th, 2016

 

GOLD ID

NA

 

BIOPROJECT

PRJNA306821

MIGS-13

Source Material identifier

SJP92

 

Project relevance

Agriculture, industry

Growth conditions and genomic DNA preparation

Flammeovirga sp. SJP92 was incubated aerobically in the modified 2216E medium (2.2% NaCl, 0.365% MgCl6H2O, 0.729% MgSO4 · 7H2O, 0.03% CaCl2 · 2H2O, 0.05% KCl, 0.042% KH2PO4, 0.005% NaBr, 0.002% SrCl · 6H2O, 0.002% Fe (NH4) Citrate, 1.326% tryptone) supplied with 0.2% agar. After incubation at 32 °C, 200 rpm for 24 h, the bacteria was collected at 13000 rpm for 30–60 min at 4 °C. The CTAB/NaCl method [10] was used for the extraction of chromosomal DNA of Flammeovirga sp. SJP92.

Genome sequencing and assembly

The genome of Flammeovirga sp. SJP92 was sequenced with MPS (massively parallel sequencing) Illumina technology. Three DNA libraries were constructed: a paired-end library with an insert size of 500 bp and two mate-pair libraries with an insert size of 5 kb. The 500 bp library and the 5 kb libraries were sequenced using an Illumina HiSeq2500 by PE125 strategy. Library construction and sequencing was performed at the Beijing Novogene Bioinformatics Technology Co., Ltd. Quality control of both paired-end and mate-pair reads were performed using in-house program. The final coverage reached 215-folds of the genome. SOAPdenovo [11, 12] was used for sequence assembly, and the final assembly yielded 123 contigs which generated a genome of 8.53 Mb.

Genome annotation

The genes of Flammeovirga sp. SJP92 was identified by NCBI Prokaryotic Genome Annotation Pipeline server online [13]. Functional predicted was performed by comparing them with sequences in RPS-BLAST against Clusters of Orthologous Groups database and pfam database [1416]. SignalP was used to predict signal peptide [17], and transmembrane helice was analyzed by TMHMM program [18]. CRISPRFinder was used for CRISPR identification [19].

Genome properties

The Flammeovirga sp. SJP92 genome has only one circular chromosome of a total size of about 8, 534, 834 bp with a 34.80% GC content (containing 123 contigs, 44 scaffolds).6519 genes were predicted, of which 6291 genes were protein-coding genes. 2660 genes (40.8%) were assigned to putative function and annotated as hypothetical proteins. And 99 RNAs (including 93 tRNAs, 5 rRNAs and 1 ncRNA), 127 pseudo genes were also identified. The properties and the statistics of the genome were summarized in Table 4, and Table 5 presented the distribution of genes into COGs functional categories. 3752 genes (57.55%) were assigned to COG functional categories, the most abundant COG category was “General function prediction only” (561 proteins) followed by “Signal transduction mechanisms” (401 proteins), “Transcription” (382 proteins), “Function unknown” (350 proteins), “Cell wall/membrane/envelope biogenesis” (347 proteins), “Inorganic ion transport and metabolism” (318 proteins), and “Carbohydrate transport and metabolism” (306 proteins).
Table 4

Genome Statistics for Flammeovirga sp. SJP92

Attribute

Value

% of Totala

Genome size (bp)

8,534,834

100.0

DNA coding (bp)

7,309,656

85.64

DNA G + C (bp)

2,970,122

34.80

DNA scaffolds

44

100.00

Total genes

6519

100.00

Protein-coding genes

6291

96.5

RNA genes

99

1.52

Pseudo genes

127

1.95

Genes in internal clusters

NA

NA

Genes with function prediction

4240

65.04

Genes assigned to COGs

3752

57.55

Genes assigned Pfam domains

3964

60.81

Genes with signal peptides

1658

25.43

Genes with transmembrane helices

1510

23.16

CRISPR repeats

1

0.01

aThe total is based on either the size of the genome in base pairs or on the total number of protein coding genes in the annotated genome

NA not available

Table 5

Number of protein coding gene of Flammeovirga sp. SJP92 associated with COG functional categories

Code

value

% age

Description

J

178

2.83

Translation, ribosomal structure and biogenesis

A

0

0

RNA processing and modification

K

382

6.07

Transcription

L

199

3.16

Replication, recombination and repair

B

2

0.03

Chromatin structure and dynamics

D

47

0.75

Cell cycle control, cell division, chromosome partitioning

V

90

1.43

Defense mechanisms

T

401

6.37

Signal transduction mechanisms

M

347

5.51

Cell wall/membrane/envelope biogenesis

N

34

0.54

Cell motility

U

80

1.27

Intracellular trafficking, secretion, and vesicular transport

O

158

2.51

Posttranslational modification, protein turnover, chaperones

C

215

3.42

Energy production and conversion

G

306

4.8

Carbohydrate transport and metabolism

E

269

4.23

Amino acid transport and metabolism

F

86

1.37

Nucleotide transport and metabolism

H

193

3.06

Coenzyme transport and metabolism

I

147

2.34

Lipid transport and metabolism

P

318

5.05

Inorganic ion transport and metabolism

Q

93

1.48

Secondary metabolites biosynthesis, transport and catabolism

R

561

8.92

General function prediction only

S

350

5.56

Function unknown

-

2539

40.35

Not in COGs

Insights from the genome sequence

Until now, only two genome sequences of the strain F. pacifica WPAGA1T and Flammeovirga sp. OC4 were available within the genus Flammeovirga . Here, a whole genome comparison with these three strains have been done (Table 6). The genome of Flammeovirga sp. SJP92 is nearly 2 Mb bigger in size than F. pacifica WPAGA1T, but almost the same as Flammeovirga sp. OC4. The G + C content of Flammeovirga sp. SJP92 (34.8%) is slightly different with F. pacifica WPAGA1T (33.8%) and Flammeovirga sp. OC4 (34.9%). The gene number of Flammeovirga sp. SJP92 is different from these two strains (6, 519 & 4, 857 & 5, 898).
Table 6

Comparison of genomes with Flammeovirga sp. SJP92, F. pacifica WPAGA1T and Flammeovirga sp. OC4

Genome Name

Flammeovirga sp.SJP92

F. pacifica WPAGA1T

Flammeovirga sp.OC4

Genome size (bp)

8, 534, 834

6, 507, 364

8, 065, 497

Gene count

6, 519

4, 857

5, 898

Protein coding

6, 291

4, 739

5, 759

Protein with function

4, 240

4, 708

5, 596

Plasmid number

0

0

0

rRNA

5

3

2

tRNA

93

68

67

GC%

34.8

33.8

34.9

Contigs

123

131

214

CRISPR repeats

1

NA

5

Genes of agarase

13

10

5

Annotation of the genome indicated that this strain possessed many agarase (14 agarases at least), which was coincident with its high agar-degrading ability. Many sulfatases were also predicted and sequence alignment of proteins indicated that these sulfatases were novel. It is an aerobic strain and the existence of genes encoding superoxide dismutase and catalase were consistent with this phenotype. Flammeovirga sp. SJP92 contained many genes related to the metabolism and transport of amino acids. Also, metabolic pathway analysis and Biolog GN2 experiments illustrated that this strain could utilize many amino acids. These evidences may reflect its ability to grow by using proteinaceous media as the carbon and energy source.

Conclusions

Flammeovirga sp. SJP92 is another strain with the genome sequence of the genus Flammeovirga together with F. pacifica WPAGA1T and Flammeovirga sp. OC4. It is an agar-degrading bacterium with efficient agarose liquefying ability and had an extracellular agarase system containing 14 agarases at least. These genomic data will provide insights into the mechanisms of how these agarases cooperation to degrade agar or other polysaccharide.

Declarations

Acknowledgments

This work was supported by the Marine Scientific Research Foundation for Public Sector Program (No. 201105027).

Authors’ contributions

LR conceived and supervised the study. QD performed the laboratory work and performed all the bioinformatics analysis with the help of HS. QD and HS drafted the manuscript and Lingwei Ruan revised the manuscript. All authors read and approved the final manuscript.

Competing interests

The authors declare that they have no competing interests.

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Authors’ Affiliations

(1)
State Key Laboratory Breeding Base of Marine Genetic Resources
(2)
Key Laboratory of Marine Genetic Resources of State Oceanic Administration, Third Institute of Oceanography, State Oceanic Administration
(3)
Key Laboratory of Marine Genetic Resources of Fujian Province
(4)
South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center

References

  1. Nakagawa Y, Hamana K, Sakane T, Yamasato K. Reclassification of Cytophaga aprica (Lewin 1969) Reichenbach 1989 in Flammeovirga gen. nov. as Flammeovirga aprica comb. nov. and of Cytophaga diffluens (ex Stanier 1940; emend. Lewin 1969) Reichenbach 1989 in Persicobacter gen. nov. as Persicobacter diffluens comb. nov. Int J Syst Bacteriol. 1997;47:220–3.View ArticleGoogle Scholar
  2. Takahashi M, Suzuki K-i, Nakagawa Y. Emendation of the genus Flammeovirga and Flammeovirga aprica with the proposal of Flammeovirga arenaria nom. rev., comb. nov. and Flammeovirga yaeyamensis sp. nov. Int J Syst Evol Microbiol. 2006;56:2095–100.View ArticlePubMedGoogle Scholar
  3. Hosoya S, Yokota A. Flammeovirga kamogawensis sp. nov., isolated from coastal seawater in Japa. Int J Syst Evol Microbiol. 2007;57:1327–30.View ArticlePubMedGoogle Scholar
  4. Han W, Gu J, Yan Q, Li J, Wu Z, Gu Q, et al. A polysaccharide-degrading marine bacterium Flammeovirga sp. MY04 and its extracellular agarase system. J Ocean Univ China. 2012;11:375–82.View ArticleGoogle Scholar
  5. Liu Y, Yi Z, Cai Y, Zeng R. Draft genome sequence of algal polysaccharides degradation bacterium, Flammeovirga sp. OC4. Mar Genomics. 2015;21:21–2.View ArticlePubMedGoogle Scholar
  6. Han W, Gu J, Cheng Y, Liu H, Li Y, Li F. A Novel Alginate Lyase (Aly5) from a Polysaccharide-Degrading Marine Bacterium Flammeovirga sp. MY04: Effects of Module Truncation to the Biochemical Characteristics, Alginate-Degradation Patterns, and Oligosaccharide-Yielding Properties. Appl Environ Microbiol. 2015;82(1):364–74.View ArticlePubMedPubMed CentralGoogle Scholar
  7. Chan Z, Wang R, Liu S, Zhao C, Yang S, Zeng R. Draft genome sequence of an agar-degrading marine bacterium Flammeovirga pacifica WPAGA1. Mar Genomics. 2015;20:23–4.View ArticlePubMedGoogle Scholar
  8. Xu H, Fu Y, Yang N, Ding Z, Lai Q, Zeng R. Flammeovirga pacifica sp. nov., isolated from deep-sea sediment. Int J Syst Evol Microbiol. 2012;62:937–41.View ArticlePubMedGoogle Scholar
  9. Field D, Garrity G, Gray T, Morrison N, Selengut J, Sterk P, et al. The minimum information about a genome sequence (MIGS) specification. Nat Biotechnol. 2008;26:541–7.View ArticlePubMedPubMed CentralGoogle Scholar
  10. Wilson K. Preparation of genomic DNA from bacteria. Curr Protoc Mol Biol. 2001 Nov;Chapter 2:Unit 2.4. doi: https://doi.org/10.1002/0471142727.mb0204s56.
  11. Li R, Li Y, Kristiansen K, Wang J. SOAP: short oligonucleotide alignment program. Bioinformatics. 2008;24:713–4.View ArticlePubMedGoogle Scholar
  12. Li R, Zhu H, Ruan J, Qian W, Fang X, Shi Z, et al. De novo assembly of human genomes with massively parallel short read sequencing. Genome Res. 2010;20:265–72.View ArticlePubMedPubMed CentralGoogle Scholar
  13. Angiuoli SV, Gussman A, Klimke W, Cochrane G, Field D, Garrity GM, et al. Toward an online repository of Standard Operating Procedures (SOPs) for (meta) genomic annotation. OMICS. 2008;12:137–41.View ArticlePubMedPubMed CentralGoogle Scholar
  14. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 1997;25:4876–82.View ArticlePubMedPubMed CentralGoogle Scholar
  15. Tatusov RL, Fedorova ND, Jackson JD, Jacobs AR, Kiryutin B, Koonin EV, et al. The COG database: an updated version includes eukaryotes. BMC Bioinformatics. 2003;4:41.View ArticlePubMedPubMed CentralGoogle Scholar
  16. Finn RD, Miller BL, Clements J, Bateman A. iPfam: a database of protein family and domain interactions found in the Protein Data Bank. Nucleic Acids Res. 2014;42:D364–D73.View ArticlePubMedGoogle Scholar
  17. Bendtsen JD, Nielsen H, von Heijne G, Brunak S. Improved prediction of signal peptides: SignalP 3. J Mol Biol. 2004;340:783–95.View ArticlePubMedGoogle Scholar
  18. Krogh A, Larsson B, Von Heijne G, Sonnhammer EL. Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes. J Mol Biol. 2001;305:567–80.View ArticlePubMedGoogle Scholar
  19. Grissa I, Vergnaud G, Pourcel C. CRISPRFinder: a web tool to identify clustered regularly interspaced short palindromic repeats. Nucleic Acids Res. 2007;35:W52–W7.View ArticlePubMedPubMed CentralGoogle Scholar
  20. Tamura K, Dudley J, Nei M, Kumar S. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol. 2007;24:1596–9.View ArticlePubMedGoogle Scholar
  21. Woese CR, Kandler O, Wheelis ML. Towards a natural system of organisms: proposal for the domains Archaea, Bacteria, and Eucarya. Proc Natl Acad Sci U S A. 1990;87:4576–9.View ArticlePubMedPubMed CentralGoogle Scholar
  22. Krieg NR, Ludwig W, Euzéby J, Whitman WB. Bergey’s Manual of Systematic Bacteriology. In: Krieg NR, Staley JT, Brown DR, Hedlund BP, Paster BJ, Ward NL, Ludwig W, Whitman WB, editors. Phylum XIV: Bacteroidetes phyl. nov, vol. 4. 2nd ed. New York: Springer; 2011. p. 25.Google Scholar
  23. Nakagawa Y, Class IV. Cytophagia class. nov. In: Krieg NR, Staley JT, Brown DR, Hedlund BP, Paster BJ, Ward NL, Ludwig W, Whitman WB, editors. Bergey’s Manual of Systematic Bacteriology, vol. 4. 2nd ed. The Bacteroidetes, Spirochaetes, Tenericutes (Mollicutes), Acidobacteria, Fibrobacteres, Fusobacteria, Dictyoglomi, Gemmatimonadetes, Lentisphaerae, Verrucomicrobia, Chlamydiae, and Planctomycetes. New York: Springer; 2010. p. 370.Google Scholar
  24. List of new names and new combinations previously effectively, but not validly, published. Int J Syst Evol Microbiol. 2012;62:1–4.Google Scholar
  25. Leadbetter ER, Order II. Cytophagales nomen novum. In: Buchanan RE, Gibbons NE, editors. Bergey’s Manual of Determinative Bacteriology. 8th ed. Baltimore: The Williams and Wilkins Co.; 1974. p. 99.Google Scholar
  26. Skerman VBD, McGowan V, Sneath PHA, Moore WEC, Moore LVH. Approved Lists. Int J Syst Bacteriol. 1980; 30:225–420.Google Scholar
  27. Yoon J, Adachi K, Park S, Kasai H, Yokota A. Aureibacter tunicatorum gen. nov., sp. nov., a marine bacterium isolated from a coral reef sea squirt, and description of Flammeovirgaceae fam. nov. Int J Syst Evol Microbiol. 2011;61:2342–7.View ArticlePubMedGoogle Scholar
  28. Ashburner M, Ball CA, Blake JA, Botstein D, Butler H, Cherry JM, et al. Gene Ontology: tool for the unification of biology. Nat Genet. 2000;25:25–9.View ArticlePubMedPubMed CentralGoogle Scholar

Copyright

© The Author(s). 2017